Novel function of beta-arrestin2 in the nucleus of mature spermatozoa Export

@article{citeulike:774304, abstract = {A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that {beta}-arrestin2, which typically shows a cytoplasmic localization owing to constitutive nuclear export, appears in the nucleus after stimulation of the G-protein-coupled odorant receptor hOR17-4. In the nucleus, {beta}-arrestin2 was involved in transcriptional regulation as shown by a Gal4-based transactivation assay. Moreover, we discovered that {beta}-arrestin2 and hOR17-4, a receptor known to have a role in sperm-egg communication, colocalize in the midpiece of mature human spermatozoa. Stimulation of hOR17-4 in spermatozoa induced PKA-dependent translocation of {beta}-arrestin2 to the nucleus and nuclear accumulation of phosphorylated MAPKs. Analysis of the interaction partners of {beta}-arrestin2 indicates that odorant receptor signaling in spermatozoa may be important for the regulation of gene expression during the early processes of fertilization. 10.1242/jcs.03046}, author = {Neuhaus, Eva M. and Mashukova, Anastasia and Barbour, Jon and Wolters, Dirk and Hatt, Hanns}, citeulike-article-id = {774304}, citeulike-linkout-0 = {http://dx.doi.org/10.1242/jcs.03046}, citeulike-linkout-1 = {http://jcs.biologists.org/cgi/content/abstract/119/15/3047}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16820410}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16820410}, day = {1}, doi = {10.1242/jcs.03046}, journal = {J Cell Sci}, keywords = {arrestin-1, sperm}, month = {August}, number = {15}, pages = {3047--3056}, posted-at = {2006-07-26 09:58:52}, priority = {2}, title = {Novel function of {beta}-arrestin2 in the nucleus of mature spermatozoa}, url = {http://dx.doi.org/10.1242/jcs.03046}, volume = {119}, year = {2006} }

TY - JOUR ID - citeulike:774304 L3 - citeulike-article-id:774304 N2 - A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that {beta}-arrestin2, which typically shows a cytoplasmic localization owing to constitutive nuclear export, appears in the nucleus after stimulation of the G-protein-coupled odorant receptor hOR17-4. In the nucleus, {beta}-arrestin2 was involved in transcriptional regulation as shown by a Gal4-based transactivation assay. Moreover, we discovered that {beta}-arrestin2 and hOR17-4, a receptor known to have a role in sperm-egg communication, colocalize in the midpiece of mature human spermatozoa. Stimulation of hOR17-4 in spermatozoa induced PKA-dependent translocation of {beta}-arrestin2 to the nucleus and nuclear accumulation of phosphorylated MAPKs. Analysis of the interaction partners of {beta}-arrestin2 indicates that odorant receptor signaling in spermatozoa may be important for the regulation of gene expression during the early processes of fertilization. 10.1242/jcs.03046 IS - 15 JF - J Cell Sci EP - 3056 TI - Novel function of {beta}-arrestin2 in the nucleus of mature spermatozoa VL - 119 SP - 3047 KW - arrestin-1 KW - sperm AU - Neuhaus, Eva AU - Mashukova, Anastasia AU - Barbour, Jon AU - Wolters, Dirk AU - Hatt, Hanns PY - 2006/08/01/ UR - http://dx.doi.org/10.1242/jcs.03046 ER -