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The anti-Helicobacter pylori effect of the extracts and the fractions obtained from Aristolochia paucinervis rhizome and leaves were studied against a reference strain of H. pylori by using the agar dilution method. Only the methanol extracts and the hexane fractions of either the rhizome or the leaves exhibited an inhibitory activity at a concentration of ≤128 μg/ml. The leaf hexane fraction APLH demonstrated a higher inhibitory activity (MIC: 4 μg/ml) than the rhizome hexane fraction APRH (MIC: 16 μg/ml), the leaf methanol extract APLM (MIC: 32 μg/ml) and the rhizome methanol extract APRM (MIC: 128 μg/ml). This inhibitory activity was confirmed for the active extracts and fractions against clinical isolates of H. pylori (n=20) for which MIC50 and MIC90 were determined.
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