Curved FtsZ protofilaments generate bending forces on liposome membranes Export

@article{Osawa_2009_EMBO, abstract = {We have created FtsZ-YFP-mts where an amphipathic helix on the C-terminus tethers FtsZ to the membrane. When incorporated inside multi-lamellar tubular liposomes, FtsZ-YFP-mts can assemble Z rings that generate a constriction force. When added to the outside of liposomes, FtsZ-YFP-mts bound and produced concave depressions, bending the membrane in the same direction as the Z ring inside liposomes. Prominent membrane tubules were then extruded at the intersections of concave depressions. We tested the effect of moving the membrane-targeting sequence (mts) from the C-terminus to the N-terminus, which is approximately 180 degrees from the C-terminal tether. When mts-FtsZ-YFP was applied to the outside of liposomes, it generated convex bulges, bending the membrane in the direction opposite to the concave depressions. We conclude that FtsZ protofilaments have a fixed direction of curvature, and the direction of membrane bending depends on which side of the bent protofilament the mts is attached to. This supports models in which the FtsZ constriction force is generated by protofilament bending.}, author = {Osawa, Masaki and Anderson, David E. and Erickson, Harold P.}, citeulike-article-id = {5841166}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/emboj.2009.277}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/emboj2009277a}, day = {24}, doi = {10.1038/emboj.2009.277}, issn = {0261-4189}, journal = {The EMBO Journal}, keywords = {bacteria, biophysics, ftsz}, month = {September}, number = {22}, pages = {3476--3484}, posted-at = {2009-10-15 23:08:19}, priority = {2}, publisher = {Nature Publishing Group}, title = {Curved FtsZ protofilaments generate bending forces on liposome membranes}, url = {http://dx.doi.org/10.1038/emboj.2009.277}, volume = {28}, year = {2009} }

TY - JOUR ID - Osawa_2009_EMBO L3 - citeulike-article-id:5841166 N2 - We have created FtsZ-YFP-mts where an amphipathic helix on the C-terminus tethers FtsZ to the membrane. When incorporated inside multi-lamellar tubular liposomes, FtsZ-YFP-mts can assemble Z rings that generate a constriction force. When added to the outside of liposomes, FtsZ-YFP-mts bound and produced concave depressions, bending the membrane in the same direction as the Z ring inside liposomes. Prominent membrane tubules were then extruded at the intersections of concave depressions. We tested the effect of moving the membrane-targeting sequence (mts) from the C-terminus to the N-terminus, which is approximately 180 degrees from the C-terminal tether. When mts-FtsZ-YFP was applied to the outside of liposomes, it generated convex bulges, bending the membrane in the direction opposite to the concave depressions. We conclude that FtsZ protofilaments have a fixed direction of curvature, and the direction of membrane bending depends on which side of the bent protofilament the mts is attached to. This supports models in which the FtsZ constriction force is generated by protofilament bending. IS - 22 JF - The EMBO Journal SN - 0261-4189 EP - 3484 TI - Curved FtsZ protofilaments generate bending forces on liposome membranes PB - Nature Publishing Group VL - 28 SP - 3476 KW - bacteria KW - biophysics KW - ftsz AU - Osawa, Masaki AU - Anderson, David AU - Erickson, Harold PY - 2009/09/24/ UR - http://dx.doi.org/10.1038/emboj.2009.277 ER -