Human immunodeficiency virus type 1 Vpr-mediated G2 arrest requires Rad17 and Hus1 and induces nuclear BRCA1 and gamma-H2AX focus formation. Export

@article{citeulike:4948640, abstract = {Eukaryotic cells have evolved a complex mechanism for sensing DNA damage during genome replication. Activation of this pathway prevents entry into mitosis to allow for either DNA repair or, in the event of irreparable damage, commitment to apoptosis. Under conditions of replication stress, the damage signal is initiated by the ataxia-telangiectasia-mutated and Rad3-related kinase ATR. We recently demonstrated that the human immunodeficiency virus type 1 (HIV-1) gene product viral protein R (Vpr) arrests infected cells in the G(2) phase via the activation of ATR. In the present study, we show that the activation of ATR by Vpr is analogous to activation by certain genotoxic agents, both mechanistically and in its downstream consequences. Specifically, we show a requirement for Rad17 and Hus1 to induce G(2) arrest as well as Vpr-induced phosphorylation of histone 2A variant X (H2AX) and formation of nuclear foci containing H2AX and breast cancer susceptibility protein 1. These results demonstrate that G(2) arrest mediated by the HIV-1 gene product Vpr utilizes the cellular signaling pathway whose physiological function is to recognize replication stress. These findings should contribute to a greater understanding of how HIV-1 manipulates the CD4(+)-lymphocyte cell cycle and apoptosis induction in the progressive CD4(+)-lymphocyte depletion characteristic of HIV-1 pathogenesis.}, author = {Zimmerman, E. S. and Chen, J. and Andersen, J. L. and Ardon, O. and Dehart, J. L. and Blackett, J. and Choudhary, S. K. and Camerini, D. and Nghiem, P. and Planelles, V.}, citeulike-article-id = {4948640}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/MCB.24.21.9286-9294.2004}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15485898}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15485898}, doi = {10.1128/MCB.24.21.9286-9294.2004}, issn = {0270-7306}, journal = {Molecular and cellular biology}, keywords = {cell, cycle, damage, dna, hiv, spliceosome, vpr}, month = {November}, number = {21}, pages = {9286--9294}, posted-at = {2009-06-24 23:52:50}, priority = {2}, title = {Human immunodeficiency virus type 1 Vpr-mediated G2 arrest requires Rad17 and Hus1 and induces nuclear BRCA1 and gamma-H2AX focus formation.}, url = {http://dx.doi.org/10.1128/MCB.24.21.9286-9294.2004}, volume = {24}, year = {2004} }

TY - JOUR ID - citeulike:4948640 L3 - citeulike-article-id:4948640 N2 - Eukaryotic cells have evolved a complex mechanism for sensing DNA damage during genome replication. Activation of this pathway prevents entry into mitosis to allow for either DNA repair or, in the event of irreparable damage, commitment to apoptosis. Under conditions of replication stress, the damage signal is initiated by the ataxia-telangiectasia-mutated and Rad3-related kinase ATR. We recently demonstrated that the human immunodeficiency virus type 1 (HIV-1) gene product viral protein R (Vpr) arrests infected cells in the G(2) phase via the activation of ATR. In the present study, we show that the activation of ATR by Vpr is analogous to activation by certain genotoxic agents, both mechanistically and in its downstream consequences. Specifically, we show a requirement for Rad17 and Hus1 to induce G(2) arrest as well as Vpr-induced phosphorylation of histone 2A variant X (H2AX) and formation of nuclear foci containing H2AX and breast cancer susceptibility protein 1. These results demonstrate that G(2) arrest mediated by the HIV-1 gene product Vpr utilizes the cellular signaling pathway whose physiological function is to recognize replication stress. These findings should contribute to a greater understanding of how HIV-1 manipulates the CD4(+)-lymphocyte cell cycle and apoptosis induction in the progressive CD4(+)-lymphocyte depletion characteristic of HIV-1 pathogenesis. IS - 21 JF - Molecular and cellular biology SN - 0270-7306 EP - 9294 TI - Human immunodeficiency virus type 1 Vpr-mediated G2 arrest requires Rad17 and Hus1 and induces nuclear BRCA1 and gamma-H2AX focus formation. VL - 24 SP - 9286 KW - cell KW - cycle KW - damage KW - dna KW - hiv KW - spliceosome KW - vpr AU - Zimmerman, ES AU - Chen, J AU - Andersen, JL AU - Ardon, O AU - Dehart, JL AU - Blackett, J AU - Choudhary, SK AU - Camerini, D AU - Nghiem, P AU - Planelles, V PY - 2004/11// UR - http://dx.doi.org/10.1128/MCB.24.21.9286-9294.2004 ER -