The conformational behaviour of phosphatidylinositol. Export

@article{citeulike:4320316, abstract = {The temperature dependence of the 1H-NMR spectrum of phosphatidylinositol (PI) in d6-dimethylsulphoxide (DMSO) shows that the hydroxy groups at C2 and at C6 of the inositol ring are internally hydrogen-bonded. This probably implies a trans/gauche conformation for the phosphate/inositol linkage. The presence of a trans phosphate-alkyl-oxygen bond is confirmed by 31P-NMR studies. If the conformation of PI in membranes is the same as that in DMSO solution, this implies that the inositol ring points out into the aqueous phase with its C1/C4 axis almost perpendicular to the membrane surface. Progress is also reported in attempts to characterise headgroup orientation and dynamics by 2H-NMR using deuterated synthetic PI, prepared by the route devised by Ward, J.G. and Young, R.C. (Tetrahedron Lett. 29 (1988) 6013-6016).}, author = {Bushby, R. J. and Byard, S. J. and Hansbro, P. M. and Reid, D. G.}, citeulike-article-id = {4320316}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/2344441}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=2344441}, day = {22}, issn = {0006-3002}, journal = {Biochimica et biophysica acta}, keywords = {headgroup, orientation, phosphoinositides}, month = {May}, number = {2}, pages = {231--236}, posted-at = {2009-04-15 20:34:01}, priority = {2}, title = {The conformational behaviour of phosphatidylinositol.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/2344441}, volume = {1044}, year = {1990} }

TY - JOUR ID - citeulike:4320316 L3 - citeulike-article-id:4320316 N2 - The temperature dependence of the 1H-NMR spectrum of phosphatidylinositol (PI) in d6-dimethylsulphoxide (DMSO) shows that the hydroxy groups at C2 and at C6 of the inositol ring are internally hydrogen-bonded. This probably implies a trans/gauche conformation for the phosphate/inositol linkage. The presence of a trans phosphate-alkyl-oxygen bond is confirmed by 31P-NMR studies. If the conformation of PI in membranes is the same as that in DMSO solution, this implies that the inositol ring points out into the aqueous phase with its C1/C4 axis almost perpendicular to the membrane surface. Progress is also reported in attempts to characterise headgroup orientation and dynamics by 2H-NMR using deuterated synthetic PI, prepared by the route devised by Ward, J.G. and Young, R.C. (Tetrahedron Lett. 29 (1988) 6013-6016). IS - 2 JF - Biochimica et biophysica acta SN - 0006-3002 EP - 236 TI - The conformational behaviour of phosphatidylinositol. VL - 1044 SP - 231 KW - headgroup KW - orientation KW - phosphoinositides AU - Bushby, RJ AU - Byard, SJ AU - Hansbro, PM AU - Reid, DG PY - 1990/05/22/ UR - http://view.ncbi.nlm.nih.gov/pubmed/2344441 ER -