General anosmia caused by a targeted disruption of the mouse olfactory cyclic nucleotide-gated cation channel. Export

@article{citeulike:6055046, abstract = {Olfactory neurons transduce the binding of odorants into membrane depolarization. Two intracellular messengers, cyclic AMP (cAMP) and inositol trisphosphate (IP3), are thought to mediate this process, with cAMP generating responses to some odorants and IP3 mediating responses to others. cAMP causes membrane depolarization by activating a cation-selective cyclic nucleotide-gated (CNG) channel. We created a mutant "knockout" mouse lacking functional olfactory CNG channels to assess the roles of different second messenger pathways in olfactory transduction. Using an electrophysiological assay, we find that excitatory responses to both cAMP- and IP3-producing odorants are undetectable in knockout mice. Our results provide direct evidence that the CNG channel subserves excitatory olfactory signal transduction, and further suggest that cAMP is the sole second messenger mediating this process.}, author = {Brunet, L. J. and Gold, G. H. and Ngai, J.}, citeulike-article-id = {6055046}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8893025}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8893025}, issn = {0896-6273}, journal = {Neuron}, keywords = {camp, ip3, olfactory}, month = {October}, number = {4}, pages = {681--693}, posted-at = {2009-11-02 02:56:47}, priority = {2}, title = {General anosmia caused by a targeted disruption of the mouse olfactory cyclic nucleotide-gated cation channel.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8893025}, volume = {17}, year = {1996} }

TY - JOUR ID - citeulike:6055046 L3 - citeulike-article-id:6055046 N2 - Olfactory neurons transduce the binding of odorants into membrane depolarization. Two intracellular messengers, cyclic AMP (cAMP) and inositol trisphosphate (IP3), are thought to mediate this process, with cAMP generating responses to some odorants and IP3 mediating responses to others. cAMP causes membrane depolarization by activating a cation-selective cyclic nucleotide-gated (CNG) channel. We created a mutant "knockout" mouse lacking functional olfactory CNG channels to assess the roles of different second messenger pathways in olfactory transduction. Using an electrophysiological assay, we find that excitatory responses to both cAMP- and IP3-producing odorants are undetectable in knockout mice. Our results provide direct evidence that the CNG channel subserves excitatory olfactory signal transduction, and further suggest that cAMP is the sole second messenger mediating this process. IS - 4 JF - Neuron SN - 0896-6273 EP - 693 TI - General anosmia caused by a targeted disruption of the mouse olfactory cyclic nucleotide-gated cation channel. VL - 17 SP - 681 KW - camp KW - ip3 KW - olfactory AU - Brunet, LJ AU - Gold, GH AU - Ngai, J PY - 1996/10// UR - http://view.ncbi.nlm.nih.gov/pubmed/8893025 ER -