A single-array preprocessing method for estimating full-resolution raw copy numbers from all Affymetrix genotyping arrays including GenomeWideSNP 5 & 6. Export

@article{citeulike:4899059, abstract = {MOTIVATION: High-resolution copy-number (CN) analysis has in recent years gained much attention, not only for the purpose of identifying CN aberrations associated with a certain phenotype, but also for identifying CN polymorphisms. In order for such studies to be successful and cost effective, the statistical methods have to be optimized. We propose a single-array preprocessing method for estimating full-resolution total CNs. It is applicable to all Affymetrix genotyping arrays, including the recent ones that also contain non-polymorphic probes. A reference signal is only needed at the last step when calculating relative CNs. RESULTS: As with our method for earlier generations of arrays, this one controls for allelic crosstalk, probe affinities and PCR fragment-length effects. Additionally, it also corrects for probe sequence effects and co-hybridization of fragments digested by multiple enzymes that takes place on the latest chips. We compare our method with Affymetrix's CN5 method and the dChip method by assessing how well they differentiate between various CN states at the full resolution and various amounts of smoothing. Although CRMA v2 is a single-array method, we observe that it performs as well as or better than alternative methods that use data from all arrays for their preprocessing. This shows that it is possible to do online analysis in large-scale projects where additional arrays are introduced over time.}, author = {Bengtsson, Henrik and Wirapati, Pratyaksha and Speed, Terence P.}, citeulike-article-id = {4899059}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/bioinformatics/btp371}, citeulike-linkout-1 = {http://bioinformatics.oxfordjournals.org/cgi/content/abstract/25/17/2149}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19535535}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19535535}, day = {1}, doi = {10.1093/bioinformatics/btp371}, issn = {1460-2059}, journal = {Bioinformatics (Oxford, England)}, keywords = {arrays, snps, sv}, month = {September}, number = {17}, pages = {2149--2156}, posted-at = {2009-08-20 19:42:56}, priority = {2}, title = {A single-array preprocessing method for estimating full-resolution raw copy numbers from all Affymetrix genotyping arrays including GenomeWideSNP 5 \& 6.}, url = {http://dx.doi.org/10.1093/bioinformatics/btp371}, volume = {25}, year = {2009} }

TY - JOUR ID - citeulike:4899059 L3 - citeulike-article-id:4899059 N2 - MOTIVATION: High-resolution copy-number (CN) analysis has in recent years gained much attention, not only for the purpose of identifying CN aberrations associated with a certain phenotype, but also for identifying CN polymorphisms. In order for such studies to be successful and cost effective, the statistical methods have to be optimized. We propose a single-array preprocessing method for estimating full-resolution total CNs. It is applicable to all Affymetrix genotyping arrays, including the recent ones that also contain non-polymorphic probes. A reference signal is only needed at the last step when calculating relative CNs. RESULTS: As with our method for earlier generations of arrays, this one controls for allelic crosstalk, probe affinities and PCR fragment-length effects. Additionally, it also corrects for probe sequence effects and co-hybridization of fragments digested by multiple enzymes that takes place on the latest chips. We compare our method with Affymetrix's CN5 method and the dChip method by assessing how well they differentiate between various CN states at the full resolution and various amounts of smoothing. Although CRMA v2 is a single-array method, we observe that it performs as well as or better than alternative methods that use data from all arrays for their preprocessing. This shows that it is possible to do online analysis in large-scale projects where additional arrays are introduced over time. IS - 17 JF - Bioinformatics (Oxford, England) SN - 1460-2059 EP - 2156 TI - A single-array preprocessing method for estimating full-resolution raw copy numbers from all Affymetrix genotyping arrays including GenomeWideSNP 5 & 6. VL - 25 SP - 2149 KW - arrays KW - snps KW - sv AU - Bengtsson, Henrik AU - Wirapati, Pratyaksha AU - Speed, Terence PY - 2009/09/01/ UR - http://dx.doi.org/10.1093/bioinformatics/btp371 ER -