Enrichment of sequencing targets from the human genome by solution hybridization Export

@article{citeulike:5958359, abstract = {To exploit fully the potential of current sequencing technologies for population-based studies, one must enrich for loci from the human genome. Here we evaluate the hybridization-based approach by using oligonucleotide capture probes in solution to enrich for approximately 3.9 Mb of sequence target. We demonstrate that the tiling probe frequency is important for generating sequence data with high uniform coverage of targets. We obtained 93\% sensitivity to detect SNPs, with a calling accuracy greater than 99\%.}, author = {Tewhey, Ryan and Nakano, Masakazu and Wang, Xiaoyun and Pena, Carlos P. and Novak, Barbara and Giuffre, Angelica and Lin, Eric and Happe, Scott and Roberts, Doug and LeProust, Emily and Topol, Eric and Harismendy, Olivier and Frazer, Kelly}, citeulike-article-id = {5958359}, citeulike-linkout-0 = {http://dx.doi.org/10.1186/gb-2009-10-10-r116}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19835619}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19835619}, day = {16}, doi = {10.1186/gb-2009-10-10-r116}, issn = {1465-6906}, journal = {Genome Biology}, keywords = {popgen, sequencing, snps}, month = {October}, number = {10}, pages = {R116+}, posted-at = {2009-11-03 01:41:15}, priority = {2}, title = {Enrichment of sequencing targets from the human genome by solution hybridization}, url = {http://dx.doi.org/10.1186/gb-2009-10-10-r116}, volume = {10}, year = {2009} }

TY - JOUR ID - citeulike:5958359 L3 - citeulike-article-id:5958359 N2 - To exploit fully the potential of current sequencing technologies for population-based studies, one must enrich for loci from the human genome. Here we evaluate the hybridization-based approach by using oligonucleotide capture probes in solution to enrich for approximately 3.9 Mb of sequence target. We demonstrate that the tiling probe frequency is important for generating sequence data with high uniform coverage of targets. We obtained 93% sensitivity to detect SNPs, with a calling accuracy greater than 99%. IS - 10 JF - Genome Biology SN - 1465-6906 TI - Enrichment of sequencing targets from the human genome by solution hybridization VL - 10 SP - R116 KW - popgen KW - sequencing KW - snps AU - Tewhey, Ryan AU - Nakano, Masakazu AU - Wang, Xiaoyun AU - Pena, Carlos AU - Novak, Barbara AU - Giuffre, Angelica AU - Lin, Eric AU - Happe, Scott AU - Roberts, Doug AU - LeProust, Emily AU - Topol, Eric AU - Harismendy, Olivier AU - Frazer, Kelly PY - 2009/10/16/ UR - http://dx.doi.org/10.1186/gb-2009-10-10-r116 ER -