The Euchromatic and Heterochromatic Landscapes Are Shaped by Antagonizing Effects of Transcription on H2A.Z Deposition Export

@article{citeulike:6057151, abstract = {A role for variant histone H2A.Z in gene expression is now well established but little is known about the mechanisms by which it operates. Using a combination of ChIP\^{a}€ ” chip, knockdown and expression profiling experiments, we show that upon gene induction, human H2A.Z associates with gene promoters and helps in recruiting the transcriptional machinery. Surprisingly, we also found that H2A.Z is randomly incorporated in the genome at low levels and that active transcription antagonizes this incorporation in transcribed regions. After cessation of transcription, random H2A.Z quickly reappears on genes, demonstrating that this incorporation utilizes an active mechanism. Within facultative heterochromatin, we observe a hyper accumulation of the variant histone, which might be due to the lack of transcription in these regions. These results show how chromatin structure and transcription can antagonize each other, therefore shaping chromatin and controlling gene expression.}, author = {Hardy, Sara and Jacques, Pierre-\'{E}tienne and G\'{e}vry, Nicolas and Forest, Audrey and Fortin, Marie-\`{E}ve and Laflamme, Liette and Gaudreau, Luc and Robert, Fran\c{c}ois}, citeulike-article-id = {6057151}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.pgen.1000687}, day = {16}, doi = {10.1371/journal.pgen.1000687}, journal = {PLoS Genet}, keywords = {chip-chip, chromatin, expression, histone}, month = {October}, number = {10}, pages = {e1000687+}, posted-at = {2009-11-02 19:05:27}, priority = {2}, publisher = {Public Library of Science}, title = {The Euchromatic and Heterochromatic Landscapes Are Shaped by Antagonizing Effects of Transcription on H2A.Z Deposition}, url = {http://dx.doi.org/10.1371/journal.pgen.1000687}, volume = {5}, year = {2009} }

TY - JOUR ID - citeulike:6057151 L3 - citeulike-article-id:6057151 N2 - A role for variant histone H2A.Z in gene expression is now well established but little is known about the mechanisms by which it operates. Using a combination of ChIP–chip, knockdown and expression profiling experiments, we show that upon gene induction, human H2A.Z associates with gene promoters and helps in recruiting the transcriptional machinery. Surprisingly, we also found that H2A.Z is randomly incorporated in the genome at low levels and that active transcription antagonizes this incorporation in transcribed regions. After cessation of transcription, random H2A.Z quickly reappears on genes, demonstrating that this incorporation utilizes an active mechanism. Within facultative heterochromatin, we observe a hyper accumulation of the variant histone, which might be due to the lack of transcription in these regions. These results show how chromatin structure and transcription can antagonize each other, therefore shaping chromatin and controlling gene expression. IS - 10 JF - PLoS Genet TI - The Euchromatic and Heterochromatic Landscapes Are Shaped by Antagonizing Effects of Transcription on H2A.Z Deposition PB - Public Library of Science VL - 5 SP - e1000687 KW - chip-chip KW - chromatin KW - expression KW - histone AU - Hardy, Sara AU - Jacques, Pierre-Étienne AU - Gévry, Nicolas AU - Forest, Audrey AU - Fortin, Marie-Ève AU - Laflamme, Liette AU - Gaudreau, Luc AU - Robert, François PY - 2009/10/16/ UR - http://dx.doi.org/10.1371/journal.pgen.1000687 ER -