Fluorescent timer : Protein that changes color with time. Export

@article{citeulike:3281806, abstract = {We generated a mutant of the red fluorescent protein drFP583. The mutant (E5) changes its fluorescence from green to red over time. The rate of color conversion is independent of protein concentration and therefore can be used to trace time-dependent expression. We used in vivo labeling with E5 to measure expression from the heat shock-dependent promoter in Caenorhabditis elegans and from the Otx-2 promoter in developing Xenopus embryos. Thus, E5 is a "fluorescent timer" that can be used to monitor both activation and down-regulation of target promoters on the whole-organism scale.AD - School of Medicine, Stanford University, Stanford, CA 94305, USA. Alexey.Terskikh@Stanford.eduFAU - Terskikh, AAU - Terskikh AFAU - Fradkov, AAU - Fradkov AFAU - Ermakova, GAU - Ermakova GFAU - Zaraisky, AAU - Zaraisky AFAU - Tan, PAU - Tan PFAU - Kajava, A VAU - Kajava AVFAU - Zhao, XAU - Zhao XFAU - Lukyanov, SAU - Lukyanov SFAU - Matz, MAU - Matz MFAU - Kim, SAU - Kim SFAU - Weissman, IAU - Weissman IFAU - Siebert, PAU - Siebert PLA - engID - 1 RO3 TW01362-01/TW/FICPT - Journal ArticleCY - UNITED STATESTA - ScienceJID - 0404511RN - 0 (Heat-Shock Proteins)RN - 0 (Luminescent Proteins)RN - 0 (Nerve Tissue Proteins)RN - 0 (Otx2 protein)RN - 0 (Trans-Activators)RN - 0 (red fluorescent protein)SB - IM}, author = {Terskikh, A. and Fradkov, A. and Ermakova, G. and Zaraisky, A. and Kajava, A. V. and Zhao, X. and Lukyanov, S. and Matz, M. and Weissman, I. and Siebert, P. and Kim, S. K. and Tan, P. B. O.}, citeulike-article-id = {3281806}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.290.5496.1585}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/290/5496/1585}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/11090358}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=11090358}, citeulike-linkout-4 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00004426}, doi = {10.1126/science.290.5496.1585}, journal = {Science}, pages = {1585--1588}, posted-at = {2009-03-02 18:30:30}, priority = {2}, title = {Fluorescent timer : Protein that changes color with time.}, url = {http://dx.doi.org/10.1126/science.290.5496.1585}, volume = {290}, year = {2000} }

TY - JOUR ID - citeulike:3281806 L3 - citeulike-article-id:3281806 N2 - We generated a mutant of the red fluorescent protein drFP583. The mutant (E5) changes its fluorescence from green to red over time. The rate of color conversion is independent of protein concentration and therefore can be used to trace time-dependent expression. We used in vivo labeling with E5 to measure expression from the heat shock-dependent promoter in Caenorhabditis elegans and from the Otx-2 promoter in developing Xenopus embryos. Thus, E5 is a "fluorescent timer" that can be used to monitor both activation and down-regulation of target promoters on the whole-organism scale.AD - School of Medicine, Stanford University, Stanford, CA 94305, USA. Alexey.Terskikh@Stanford.eduFAU - Terskikh, AAU - Terskikh AFAU - Fradkov, AAU - Fradkov AFAU - Ermakova, GAU - Ermakova GFAU - Zaraisky, AAU - Zaraisky AFAU - Tan, PAU - Tan PFAU - Kajava, A VAU - Kajava AVFAU - Zhao, XAU - Zhao XFAU - Lukyanov, SAU - Lukyanov SFAU - Matz, MAU - Matz MFAU - Kim, SAU - Kim SFAU - Weissman, IAU - Weissman IFAU - Siebert, PAU - Siebert PLA - engID - 1 RO3 TW01362-01/TW/FICPT - Journal ArticleCY - UNITED STATESTA - ScienceJID - 0404511RN - 0 (Heat-Shock Proteins)RN - 0 (Luminescent Proteins)RN - 0 (Nerve Tissue Proteins)RN - 0 (Otx2 protein)RN - 0 (Trans-Activators)RN - 0 (red fluorescent protein)SB - IM JF - Science EP - 1588 TI - Fluorescent timer : Protein that changes color with time. VL - 290 SP - 1585 AU - Terskikh, A AU - Fradkov, A AU - Ermakova, G AU - Zaraisky, A AU - Kajava, AV AU - Zhao, X AU - Lukyanov, S AU - Matz, M AU - Weissman, I AU - Siebert, P AU - Kim, SK AU - Tan, PBO PY - 2000/// UR - http://dx.doi.org/10.1126/science.290.5496.1585 ER -