Intensification of labelings of the immunogold silver staining method by gold toning.

@article{citeulike:2690966, abstract = {We evaluated the applicability of the gold toning procedure to the immunogold silver staining method using monoclonal antibody against dopamine. Immunolabeling was examined in the rat substantia nigra at light and electron microscopic levels. Vibratome sections of fixed midbrains were incubated with anti-dopamine antiserum and then with 5 nm colloidal gold bound to goat anti-mouse immunoglobulin. Silver staining of these sections produced a light brown immunolabeling. After the sections were processed by gold toning, the labeling became intense black. At a light microscopic level, these high contrast signals were retained after the sections were osmicated and embedded in Epon. At an electron microscopic level, signal-to-noise ratio was high, and the positive staining could easily be verified at a low-power magnification. The technique described here, the gold-toned immunogold silver staining method, provides high contrast signals and is much more sensitive than immunogold silver staining alone. This method, therefore, has great potential for use in immunohistochemical analysis of the central nervous system.}, address = {D\'{e}partement de Cytologie, Universit\'{e} Pierre et Marie Curie, France.}, author = {Arai, R. and Geffard, M. and Calas, A. }, citeulike-article-id = {2690966}, issn = {0361-9230}, journal = {Brain research bulletin}, keywords = {addons-metal, metallization, metallization-ag, metallization-au, nanogold, origami}, month = {February}, number = {2}, pages = {343--345}, posted-at = {2008-04-19 18:04:50}, priority = {2}, title = {Intensification of labelings of the immunogold silver staining method by gold toning.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1375863}, volume = {28}, year = {1992} }

TY - JOUR ID - citeulike:2690966 L3 - citeulike-article-id:2690966 TI - Intensification of labelings of the immunogold silver staining method by gold toning. JF - Brain research bulletin VL - 28 IS - 2 SP - 343 EP - 345 AD - Département de Cytologie, Université Pierre et Marie Curie, France. SN - 0361-9230 N2 - We evaluated the applicability of the gold toning procedure to the immunogold silver staining method using monoclonal antibody against dopamine. Immunolabeling was examined in the rat substantia nigra at light and electron microscopic levels. Vibratome sections of fixed midbrains were incubated with anti-dopamine antiserum and then with 5 nm colloidal gold bound to goat anti-mouse immunoglobulin. Silver staining of these sections produced a light brown immunolabeling. After the sections were processed by gold toning, the labeling became intense black. At a light microscopic level, these high contrast signals were retained after the sections were osmicated and embedded in Epon. At an electron microscopic level, signal-to-noise ratio was high, and the positive staining could easily be verified at a low-power magnification. The technique described here, the gold-toned immunogold silver staining method, provides high contrast signals and is much more sensitive than immunogold silver staining alone. This method, therefore, has great potential for use in immunohistochemical analysis of the central nervous system. KW - addons-metal KW - metallization KW - metallization-ag KW - metallization-au KW - nanogold KW - origami AU - Arai, R AU - Geffard, M AU - Calas, A PY - 1992/02// UR - http://view.ncbi.nlm.nih.gov/pubmed/1375863 ER -