Genome-Wide Analysis in Vivo of Translation with Nucleotide Resolution Using Ribosome Profiling Export

@article{citeulike:4042344, abstract = {Techniques for systematically monitoring protein translation have lagged far behind methods for measuring messenger RNA (mRNA) levels. Here, we present a ribosome-profiling strategy that is based on the deep sequencing of ribosome-protected mRNA fragments and enables genome-wide investigation of translation with subcodon resolution. We used this technique to monitor translation in budding yeast under both rich and starvation conditions. These studies defined the protein sequences being translated and found extensive translational control in both determining absolute protein abundance and responding to environmental stress. We also observed distinct phases during translation that involve a large decrease in ribosome density going from early to late peptide elongation as well as widespread regulated initiation at non-adenine-uracil-guanine (AUG) codons. Ribosome profiling is readily adaptable to other organisms, making high-precision investigation of protein translation experimentally accessible. 10.1126/science.1168978}, author = {Ingolia, Nicholas T. and Ghaemmaghami, Sina and Newman, John R. S. and Weissman, Jonathan S.}, citeulike-article-id = {4042344}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1168978}, citeulike-linkout-1 = {http://www.sciencemag.org/content/324/5924/218.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/324/5924/218.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/1168978//}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/19213877}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=19213877}, day = {10}, doi = {10.1126/science.1168978}, issn = {1095-9203}, journal = {Science}, keywords = {genome\_wide, translation\_rate}, month = {April}, number = {5924}, pages = {218--223}, posted-at = {2009-06-03 19:14:40}, priority = {2}, title = {Genome-Wide Analysis in Vivo of Translation with Nucleotide Resolution Using Ribosome Profiling}, url = {http://dx.doi.org/10.1126/science.1168978}, volume = {324}, year = {2009} }

TY - JOUR ID - citeulike:4042344 L3 - citeulike-article-id:4042344 N2 - Techniques for systematically monitoring protein translation have lagged far behind methods for measuring messenger RNA (mRNA) levels. Here, we present a ribosome-profiling strategy that is based on the deep sequencing of ribosome-protected mRNA fragments and enables genome-wide investigation of translation with subcodon resolution. We used this technique to monitor translation in budding yeast under both rich and starvation conditions. These studies defined the protein sequences being translated and found extensive translational control in both determining absolute protein abundance and responding to environmental stress. We also observed distinct phases during translation that involve a large decrease in ribosome density going from early to late peptide elongation as well as widespread regulated initiation at non-adenine-uracil-guanine (AUG) codons. Ribosome profiling is readily adaptable to other organisms, making high-precision investigation of protein translation experimentally accessible. 10.1126/science.1168978 IS - 5924 JF - Science SN - 1095-9203 EP - 223 TI - Genome-Wide Analysis in Vivo of Translation with Nucleotide Resolution Using Ribosome Profiling VL - 324 SP - 218 KW - genome_wide KW - translation_rate AU - Ingolia, Nicholas AU - Ghaemmaghami, Sina AU - Newman, John AU - Weissman, Jonathan PY - 2009/04/10/ UR - http://dx.doi.org/10.1126/science.1168978 ER -