Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Export

@article{Schena1995, abstract = {A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.}, address = {Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305, USA.}, author = {Schena, M. and Shalon, D. and Davis, R. W. and Brown, P. O.}, citeulike-article-id = {269364}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.270.5235.467}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/270/5235/467}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/7569999}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=7569999}, day = {20}, doi = {10.1126/science.270.5235.467}, issn = {0036-8075}, journal = {Science (New York, N.Y.)}, keywords = {microarray}, month = {October}, number = {5235}, pages = {467--470}, posted-at = {2008-04-16 22:40:28}, priority = {2}, title = {Quantitative monitoring of gene expression patterns with a complementary DNA microarray.}, url = {http://dx.doi.org/10.1126/science.270.5235.467}, volume = {270}, year = {1995} }

TY - JOUR ID - Schena1995 L3 - citeulike-article-id:269364 N2 - A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization. IS - 5235 JF - Science (New York, N.Y.) SN - 0036-8075 AD - Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305, USA. EP - 470 TI - Quantitative monitoring of gene expression patterns with a complementary DNA microarray. VL - 270 SP - 467 KW - microarray AU - Schena, M AU - Shalon, D AU - Davis, RW AU - Brown, PO PY - 1995/10/20/ UR - http://dx.doi.org/10.1126/science.270.5235.467 ER -