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Plant Molecular Biology Reporter, Vol. 12, No. 4. (1 December 1994), pp. 321-331, doi:10.1007/bf02669275 Key: citeulike:11598837
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A procedure is presented for the detection of mRNA in whole-mount preparations of young Arabidopsis seedlings using digoxigenin (DIG)-labeled RNA probes. It includes tissue preservation with formaldehyde, permeabilization with polyoxyethy lenesorbitan (Tween 20), DMSO, and heptane. Hybridization signal is detected using colloidal gold or alkaline-phosphatase-conjugated anti-DIG antibodies.
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