Posttranslational Mechanisms Regulate the Mammalian Circadian Clock Export

@article{citeulike:2335005, abstract = {We have examined posttranslational regulation of clock proteins in mouse liver in vivo. The mouse PERIOD proteins (mPER1 and mPER2), CLOCK, and BMAL1 undergo robust circadian changes in phosphorylation. These proteins, the cryptochromes (mCRY1 and mCRY2), and casein kinase I epsilon (CKI ) form multimeric complexes that are bound to DNA during negative transcriptional feedback. CLOCK:BMAL1 heterodimers remain bound to DNA over the circadian cycle. The temporal increase in mPER abundance controls the negative feedback interactions. Analysis of clock proteins in mCRY-deficient mice shows that the mCRYs are necessary for stabilizing phosphorylated mPER2 and for the nuclear accumulation of mPER1, mPER2, and CKI . We also provide in vivo evidence that casein kinase I delta is a second clock relevant kinase.}, author = {Lee, Choogon and Etchegaray, Jean-Pierre and Cagampang, Felino R. A. and Loudon, Andrew S. I. and Reppert, Steven M.}, citeulike-article-id = {2335005}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0092-8674(01)00610-9}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0092867401006109}, citeulike-linkout-2 = {http://www.sciencedirect.com/science/article/B6WSN-44VDTRF-7/2/5525e6a459e3bf2badc0008ba9536742}, day = {28}, doi = {10.1016/S0092-8674(01)00610-9}, issn = {00928674}, journal = {Cell}, keywords = {cry1, cry2, mouse, posttranslational}, month = {December}, number = {7}, pages = {855--867}, posted-at = {2009-11-02 15:41:13}, priority = {2}, title = {Posttranslational Mechanisms Regulate the Mammalian Circadian Clock}, url = {http://dx.doi.org/10.1016/S0092-8674(01)00610-9}, volume = {107}, year = {2001} }

TY - JOUR ID - citeulike:2335005 L3 - citeulike-article-id:2335005 N2 - We have examined posttranslational regulation of clock proteins in mouse liver in vivo. The mouse PERIOD proteins (mPER1 and mPER2), CLOCK, and BMAL1 undergo robust circadian changes in phosphorylation. These proteins, the cryptochromes (mCRY1 and mCRY2), and casein kinase I epsilon (CKI ) form multimeric complexes that are bound to DNA during negative transcriptional feedback. CLOCK:BMAL1 heterodimers remain bound to DNA over the circadian cycle. The temporal increase in mPER abundance controls the negative feedback interactions. Analysis of clock proteins in mCRY-deficient mice shows that the mCRYs are necessary for stabilizing phosphorylated mPER2 and for the nuclear accumulation of mPER1, mPER2, and CKI . We also provide in vivo evidence that casein kinase I delta is a second clock relevant kinase. IS - 7 JF - Cell SN - 00928674 EP - 867 TI - Posttranslational Mechanisms Regulate the Mammalian Circadian Clock VL - 107 SP - 855 KW - cry1 KW - cry2 KW - mouse KW - posttranslational AU - Lee, Choogon AU - Etchegaray, Jean-Pierre AU - Cagampang, Felino AU - Loudon, Andrew AU - Reppert, Steven PY - 2001/12/28/ UR - http://dx.doi.org/10.1016/S0092-8674(01)00610-9 ER -