PIF3 is a repressor of chloroplast development Export

@article{citeulike:4510238, abstract = {10.1073/pnas.0811684106 The phytochrome-interacting factor PIF3 has been proposed to act as a positive regulator of chloroplast development. Here, we show that the mutant has a phenotype that is similar to the mutant, lacking the repressor of chloroplast development PIF1, and that a double mutant has an additive phenotype in all respects. The mutants showed elevated protochlorophyllide levels in the dark, and etioplasts of mutants contained smaller prolamellar bodies and more prothylakoid membranes than corresponding wild-type seedlings, similar to previous reports of constitutive photomorphogenic mutants. Consistent with this observation, , , and showed reduced hypocotyl elongation and increased cotyledon opening in the dark. Transfer of 4-d-old dark-grown seedlings to white light resulted in more chlorophyll synthesis in mutants over the first 2 h, and analysis of gene expression in dark-grown mutants indicated that key tetrapyrrole regulatory genes such as encoding the rate-limiting step in tetrapyrrole synthesis were already elevated 2 d after germination. Circadian regulation of in the dark also showed reduced amplitude and a shorter, variable period in the mutants, whereas expression of the core clock components , , and was largely unaffected. Expression of both and was circadian regulated in dark-grown seedlings. PIF1 and PIF3 are proposed to be negative regulators that function to integrate light and circadian control in the regulation of chloroplast development.}, author = {Stephenson, Patrick G. and Fankhauser, Christian and Terry, Matthew J.}, citeulike-article-id = {4510238}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0811684106}, citeulike-linkout-1 = {http://www.pnas.org/content/106/18/7654.long.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/106/18/7654.long.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19380736}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19380736}, day = {5}, doi = {10.1073/pnas.0811684106}, journal = {Proceedings of the National Academy of Sciences}, keywords = {pif3}, month = {May}, number = {18}, pages = {7654--7659}, posted-at = {2009-05-13 16:06:23}, priority = {5}, title = {PIF3 is a repressor of chloroplast development}, url = {http://dx.doi.org/10.1073/pnas.0811684106}, volume = {106}, year = {2009} }

TY - JOUR ID - citeulike:4510238 L3 - citeulike-article-id:4510238 N2 - 10.1073/pnas.0811684106 The phytochrome-interacting factor PIF3 has been proposed to act as a positive regulator of chloroplast development. Here, we show that the mutant has a phenotype that is similar to the mutant, lacking the repressor of chloroplast development PIF1, and that a double mutant has an additive phenotype in all respects. The mutants showed elevated protochlorophyllide levels in the dark, and etioplasts of mutants contained smaller prolamellar bodies and more prothylakoid membranes than corresponding wild-type seedlings, similar to previous reports of constitutive photomorphogenic mutants. Consistent with this observation, , , and showed reduced hypocotyl elongation and increased cotyledon opening in the dark. Transfer of 4-d-old dark-grown seedlings to white light resulted in more chlorophyll synthesis in mutants over the first 2 h, and analysis of gene expression in dark-grown mutants indicated that key tetrapyrrole regulatory genes such as encoding the rate-limiting step in tetrapyrrole synthesis were already elevated 2 d after germination. Circadian regulation of in the dark also showed reduced amplitude and a shorter, variable period in the mutants, whereas expression of the core clock components , , and was largely unaffected. Expression of both and was circadian regulated in dark-grown seedlings. PIF1 and PIF3 are proposed to be negative regulators that function to integrate light and circadian control in the regulation of chloroplast development. IS - 18 JF - Proceedings of the National Academy of Sciences EP - 7659 TI - PIF3 is a repressor of chloroplast development VL - 106 SP - 7654 KW - pif3 AU - Stephenson, Patrick AU - Fankhauser, Christian AU - Terry, Matthew PY - 2009/05/05/ UR - http://dx.doi.org/10.1073/pnas.0811684106 ER -