A Functional Genomics Approach Reveals CHE as a Component of the Arabidopsis Circadian Clock Export

@article{citeulike:4821895, abstract = {Transcriptional feedback loops constitute the molecular circuitry of the plant circadian clock. In Arabidopsis, a core loop is established between CCA1 and TOC1. Although CCA1 directly represses TOC1, the TOC1 protein has no DNA binding domains, which suggests that it cannot directly regulate CCA1. We established a functional genomic strategy that led to the identification of CHE, a TCP transcription factor that binds specifically to the CCA1 promoter. CHE is a clock component partially redundant with LHY in the repression of CCA1. The expression of CHE is regulated by CCA1, thus adding a CCA1/CHE feedback loop to the Arabidopsis circadian network. Because CHE and TOC1 interact, and CHE binds to the CCA1 promoter, a molecular linkage between TOC1 and CCA1 gene regulation is established. 10.1126/science.1167206}, author = {Pruneda-Paz, Jose L. and Breton, Ghislain and Para, Alessia and Kay, Steve A.}, citeulike-article-id = {4821895}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1167206}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/323/5920/1481}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19286557}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19286557}, day = {13}, doi = {10.1126/science.1167206}, journal = {Science}, keywords = {cca1, che, promoter, toc1}, month = {March}, number = {5920}, pages = {1481--1485}, posted-at = {2009-09-22 14:11:37}, priority = {0}, title = {A Functional Genomics Approach Reveals CHE as a Component of the Arabidopsis Circadian Clock}, url = {http://dx.doi.org/10.1126/science.1167206}, volume = {323}, year = {2009} }

TY - JOUR ID - citeulike:4821895 L3 - citeulike-article-id:4821895 N2 - Transcriptional feedback loops constitute the molecular circuitry of the plant circadian clock. In Arabidopsis, a core loop is established between CCA1 and TOC1. Although CCA1 directly represses TOC1, the TOC1 protein has no DNA binding domains, which suggests that it cannot directly regulate CCA1. We established a functional genomic strategy that led to the identification of CHE, a TCP transcription factor that binds specifically to the CCA1 promoter. CHE is a clock component partially redundant with LHY in the repression of CCA1. The expression of CHE is regulated by CCA1, thus adding a CCA1/CHE feedback loop to the Arabidopsis circadian network. Because CHE and TOC1 interact, and CHE binds to the CCA1 promoter, a molecular linkage between TOC1 and CCA1 gene regulation is established. 10.1126/science.1167206 IS - 5920 JF - Science EP - 1485 TI - A Functional Genomics Approach Reveals CHE as a Component of the Arabidopsis Circadian Clock VL - 323 SP - 1481 KW - cca1 KW - che KW - promoter KW - toc1 AU - Pruneda-Paz, Jose AU - Breton, Ghislain AU - Para, Alessia AU - Kay, Steve PY - 2009/03/13/ UR - http://dx.doi.org/10.1126/science.1167206 ER -