The circadian clock controls the expression pattern of the circadian input photoreceptor, phytochrome B Export

@article{citeulike:4921587, abstract = {Developmental and physiological responses are regulated by light throughout the entire life cycle of higher plants. To sense changes in the light environment, plants have developed various photoreceptors, including the red/far-red light-absorbing phytochromes and blue light-absorbing cryptochromes. A wide variety of physiological responses, including most light responses, also are modulated by circadian rhythms that are generated by an endogenous oscillator, the circadian clock. To provide information on local time, circadian clocks are synchronized and entrained by environmental time cues, of which light is among the most important. Light-driven entrainment of the circadian clock has been shown to be mediated by phytochrome A (phyA), phytochrome B (phyB), and cryptochromes 1 and 2, thus affirming the roles of these photoreceptors as input regulators to the plant circadian clock. Here we show that the expression of ∷ reporter genes containing the promoter and 5′ untranslated region of the tobacco or genes fused to the luciferase () gene exhibit robust circadian oscillations in transgenic plants. We demonstrate that the abundance of RNA retains this circadian regulation and use a PHYB∷Luc fusion protein to show that the rate of PHYB synthesis is also rhythmic. The abundance of bulk PHYB protein, however, exhibits only weak circadian rhythmicity, if any. These data suggest that photoreceptor gene expression patterns may be significant in the daily regulation of plant physiology and indicate an unexpectedly intimate relationship between the components of the input pathway and the putative circadian clock mechanism in higher plants.}, author = {Bogn\'{a}r, L\'{a}szl\'{o} K. and Hall, Anthony and \'{A}d\'{a}m, \'{E}va and Thain, Simon C. and Nagy, Ferenc and Millar, Andrew J.}, citeulike-article-id = {4921587}, citeulike-linkout-0 = {http://www.pnas.org/content/96/25/14652.long.abstract}, citeulike-linkout-1 = {http://www.pnas.org/content/96/25/14652.long.full.pdf}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/10588760}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=10588760}, day = {7}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {phyb, phytochrome}, month = {December}, number = {25}, pages = {14652--14657}, posted-at = {2009-06-22 10:48:53}, priority = {2}, title = {The circadian clock controls the expression pattern of the circadian input photoreceptor, phytochrome B}, url = {http://www.pnas.org/content/96/25/14652.long.abstract}, volume = {96}, year = {1999} }

TY - JOUR ID - citeulike:4921587 L3 - citeulike-article-id:4921587 N2 - Developmental and physiological responses are regulated by light throughout the entire life cycle of higher plants. To sense changes in the light environment, plants have developed various photoreceptors, including the red/far-red light-absorbing phytochromes and blue light-absorbing cryptochromes. A wide variety of physiological responses, including most light responses, also are modulated by circadian rhythms that are generated by an endogenous oscillator, the circadian clock. To provide information on local time, circadian clocks are synchronized and entrained by environmental time cues, of which light is among the most important. Light-driven entrainment of the circadian clock has been shown to be mediated by phytochrome A (phyA), phytochrome B (phyB), and cryptochromes 1 and 2, thus affirming the roles of these photoreceptors as input regulators to the plant circadian clock. Here we show that the expression of ∷ reporter genes containing the promoter and 5′ untranslated region of the tobacco or genes fused to the luciferase () gene exhibit robust circadian oscillations in transgenic plants. We demonstrate that the abundance of RNA retains this circadian regulation and use a PHYB∷Luc fusion protein to show that the rate of PHYB synthesis is also rhythmic. The abundance of bulk PHYB protein, however, exhibits only weak circadian rhythmicity, if any. These data suggest that photoreceptor gene expression patterns may be significant in the daily regulation of plant physiology and indicate an unexpectedly intimate relationship between the components of the input pathway and the putative circadian clock mechanism in higher plants. IS - 25 JF - Proceedings of the National Academy of Sciences of the United States of America EP - 14657 TI - The circadian clock controls the expression pattern of the circadian input photoreceptor, phytochrome B VL - 96 SP - 14652 KW - phyb KW - phytochrome AU - Bognár, László AU - Hall, Anthony AU - Ádám, Éva AU - Thain, Simon AU - Nagy, Ferenc AU - Millar, Andrew PY - 1999/12/07/ UR - http://www.pnas.org/content/96/25/14652.long.abstract ER -