Patterns of Expression and Normalized Levels of the Five Arabidopsis Phytochromes Export

@article{citeulike:5249552, abstract = {Using monoclonal antibodies specific for each apoprotein and full-length purified apoprotein standards, the levels of the five Arabidopsis phytochromes and their patterns of expression in seedlings and mature plants and under different light conditions have been characterized. Phytochrome levels are normalized to the DNA content of the various tissue extracts to approximate normalization to the number of cells in the tissue. One phytochrome, phytochrome A, is highly light labile. The other four phytochromes are much more light stable, although among these, phytochromes B and C are reduced 4- to 5-fold in red- or white-light-grown seedlings compared with dark-grown seedlings. The total amount of extractable phytochrome is 23-fold lower in light-grown than dark-grown tissues, and the percent ratios of the five phytochromes, A:B:C:D:E, are measured as 85:10:2:1.5:1.5 in etiolated seedlings and 5:40:15:15:25 in seedlings grown in continuous white light. The four light-stable phytochromes are present at nearly unchanging levels throughout the course of development of mature rosette and reproductive-stage plants and are present in leaves, stems, roots, and flowers. Phytochrome protein expression patterns over the course of seed germination and under diurnal and circadian light cycles are also characterized. Little cycling in response to photoperiod is observed, and this very low amplitude cycling of some phytochrome proteins is out of phase with previously reported cycling of PHY mRNA levels. These studies indicate that, with the exception of phytochrome A, the family of phytochrome photoreceptors in Arabidopsis constitutes a quite stable and very broadly distributed array of sensory molecules. 10.1104/pp.005389}, author = {Sharrock, Robert A. and Clack, Ted}, citeulike-article-id = {5249552}, citeulike-linkout-0 = {http://dx.doi.org/10.1104/pp.005389}, citeulike-linkout-1 = {http://www.plantphysiol.org/cgi/content/abstract/130/1/442}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/12226523}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=12226523}, day = {1}, doi = {10.1104/pp.005389}, journal = {Plant Physiol.}, keywords = {antibodies, phya, phyb, phyc, phyd, phye, phytochrome}, month = {September}, number = {1}, pages = {442--456}, posted-at = {2009-07-24 11:03:00}, priority = {0}, title = {Patterns of Expression and Normalized Levels of the Five Arabidopsis Phytochromes}, url = {http://dx.doi.org/10.1104/pp.005389}, volume = {130}, year = {2002} }

TY - JOUR ID - citeulike:5249552 L3 - citeulike-article-id:5249552 N2 - Using monoclonal antibodies specific for each apoprotein and full-length purified apoprotein standards, the levels of the five Arabidopsis phytochromes and their patterns of expression in seedlings and mature plants and under different light conditions have been characterized. Phytochrome levels are normalized to the DNA content of the various tissue extracts to approximate normalization to the number of cells in the tissue. One phytochrome, phytochrome A, is highly light labile. The other four phytochromes are much more light stable, although among these, phytochromes B and C are reduced 4- to 5-fold in red- or white-light-grown seedlings compared with dark-grown seedlings. The total amount of extractable phytochrome is 23-fold lower in light-grown than dark-grown tissues, and the percent ratios of the five phytochromes, A:B:C:D:E, are measured as 85:10:2:1.5:1.5 in etiolated seedlings and 5:40:15:15:25 in seedlings grown in continuous white light. The four light-stable phytochromes are present at nearly unchanging levels throughout the course of development of mature rosette and reproductive-stage plants and are present in leaves, stems, roots, and flowers. Phytochrome protein expression patterns over the course of seed germination and under diurnal and circadian light cycles are also characterized. Little cycling in response to photoperiod is observed, and this very low amplitude cycling of some phytochrome proteins is out of phase with previously reported cycling of PHY mRNA levels. These studies indicate that, with the exception of phytochrome A, the family of phytochrome photoreceptors in Arabidopsis constitutes a quite stable and very broadly distributed array of sensory molecules. 10.1104/pp.005389 IS - 1 JF - Plant Physiol. EP - 456 TI - Patterns of Expression and Normalized Levels of the Five Arabidopsis Phytochromes VL - 130 SP - 442 KW - antibodies KW - phya KW - phyb KW - phyc KW - phyd KW - phye KW - phytochrome AU - Sharrock, Robert AU - Clack, Ted PY - 2002/09/01/ UR - http://dx.doi.org/10.1104/pp.005389 ER -