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The Arabidopsis HOS1 gene negatively regulates cold signal transduction and encodes a RING finger protein that displays cold-regulated nucleoâcytoplasmic partitioning Export

Genes & Development, Vol. 15, No. 7. (1 April 2001), pp. 912-924.

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cbfs cold flc hos1

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10.1101/gad.866801 Low temperature is one of the most important environmental stimuli that control gene transcription programs and development in plants. In, the locus is a key negative regulator of low temperature-responsive gene transcription. The recessive mutation causes enhanced induction of the transcription factors by low temperature as well as of their downstream cold-responsive genes. The mutant plants flower early, and this correlates with a low level of gene expression. The gene was isolated through positional cloning. encodes a novel protein with a RING finger motif near the amino terminus. is ubiquitously expressed in all plant tissues. HOS1âGFP translational fusion studies reveal that HOS1 protein resides in the cytoplasm at normal growth temperatures. However, in response to low temperature treatments, HOS1 accumulates in the nucleus. Ectopic expression of in wild-type plants causes cosuppression of expression and mimics the mutant phenotypes.


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