Circadian amplitude of cryptochrome 1 is modulated by mRNA stability regulation via cytoplasmic hnRNP D oscillation Export

@article{citeulike:6056652, abstract = {The mammalian circadian rhythm is observed not only at the suprachiasmatic nucleus, a master pacemaker, but also throughout the peripheral tissues. Its conserved molecular basis has been thought to consist of intracellular transcriptional feedback loops of key clock genes. However, little is known about post-transcriptional regulation of these genes. In the present study, we investigated the role of the 3'-untranslated region of the mouse cryptochrome 1 (mcry1) gene at the post-transcriptional level. Mature mcry1 mRNA has a 610-nucleotide 3'UTR, and mediates its own degradation. The middle part of the 3'UTR contains a destabilizing cis-acting element. The deletion of this element led to a dramatic increase in mRNA stability, and heterogeneous nuclear ribonucleoprotein (hnRNP) D was identified as an RNA-binding protein responsible for this effect. Cytoplasmic hnRNP D levels displayed a reciprocal pattern compared to mcry1 oscillation. Knockdown of hnRNP D stabilized mcry1 mRNA and resulted in enhancement of the oscillation amplitude and slight delay of the phase. Our results suggest that hnRNP D plays a role as a fine regulator, contributing to the mcry1 mRNA turnover rate and the modulation of circadian rhythm. 10.1128/MCB.01154-09}, author = {Woo, Kyung-Chul and Ha, Dae-Cheong and Lee, Kyung-Ha and Kim, Do-Yeon and Kim, Tae-Don and Kim, Kyong-Tai}, citeulike-article-id = {6056652}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/MCB.01154-09}, citeulike-linkout-1 = {http://mcb.asm.org/content/MCB.01154-09v1/.abstract}, citeulike-linkout-2 = {http://mcb.asm.org/content/MCB.01154-09v1/.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19858287}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19858287}, day = {26}, doi = {10.1128/MCB.01154-09}, journal = {Mol. Cell. Biol.}, keywords = {cry1, mouse, posttranslational}, month = {October}, pages = {MCB.01154-09+}, posted-at = {2009-11-02 15:28:42}, priority = {2}, title = {Circadian amplitude of cryptochrome 1 is modulated by mRNA stability regulation via cytoplasmic hnRNP D oscillation}, url = {http://dx.doi.org/10.1128/MCB.01154-09}, year = {2009} }

TY - JOUR ID - citeulike:6056652 L3 - citeulike-article-id:6056652 N2 - The mammalian circadian rhythm is observed not only at the suprachiasmatic nucleus, a master pacemaker, but also throughout the peripheral tissues. Its conserved molecular basis has been thought to consist of intracellular transcriptional feedback loops of key clock genes. However, little is known about post-transcriptional regulation of these genes. In the present study, we investigated the role of the 3'-untranslated region of the mouse cryptochrome 1 (mcry1) gene at the post-transcriptional level. Mature mcry1 mRNA has a 610-nucleotide 3'UTR, and mediates its own degradation. The middle part of the 3'UTR contains a destabilizing cis-acting element. The deletion of this element led to a dramatic increase in mRNA stability, and heterogeneous nuclear ribonucleoprotein (hnRNP) D was identified as an RNA-binding protein responsible for this effect. Cytoplasmic hnRNP D levels displayed a reciprocal pattern compared to mcry1 oscillation. Knockdown of hnRNP D stabilized mcry1 mRNA and resulted in enhancement of the oscillation amplitude and slight delay of the phase. Our results suggest that hnRNP D plays a role as a fine regulator, contributing to the mcry1 mRNA turnover rate and the modulation of circadian rhythm. 10.1128/MCB.01154-09 JF - Mol. Cell. Biol. TI - Circadian amplitude of cryptochrome 1 is modulated by mRNA stability regulation via cytoplasmic hnRNP D oscillation SP - MCB.01154-09 KW - cry1 KW - mouse KW - posttranslational AU - Woo, Kyung-Chul AU - Ha, Dae-Cheong AU - Lee, Kyung-Ha AU - Kim, Do-Yeon AU - Kim, Tae-Don AU - Kim, Kyong-Tai PY - 2009/10/26/ UR - http://dx.doi.org/10.1128/MCB.01154-09 ER -