Strategy for identification of Bacillus cereus and Bacillus thuringiensis strains closely related to Bacillus anthracis. Export

@article{citeulike:2089932, abstract = {Bacillus cereus strains that are genetically closely related to B. anthracis can display anthrax-like virulence traits (A. R. Hoffmaster et al., Proc. Natl. Acad. Sci. USA 101:8449-8454, 2004). Hence, approaches that rapidly identify these "near neighbors" are of great interest for the study of B. anthracis virulence mechanisms, as well as to prevent the use of such strains for B. anthracis-based bioweapon development. Here, a strategy is proposed for the identification of near neighbors of B. anthracis based on single nucleotide polymorphisms (SNP) in the 16S-23S rRNA intergenic spacer (ITS) containing tRNA genes, characteristic of B. anthracis. By using restriction site insertion-PCR (RSI-PCR) the presence of two SNP typical of B. anthracis was screened in 126 B. cereus group strains of different origin. Two B. cereus strains and one B. thuringiensis strain showed RSI-PCR profiles identical to that of B. anthracis. The sequencing of the entire ITS containing tRNA genes revealed two of the strains to be identical to B. anthracis. The strict relationship with B. anthracis was confirmed by multilocus sequence typing (MLST) of four other independent loci: cerA, plcR, AC-390, and SG-749. The relationship to B. anthracis of the three strains described by MLST was comparable and even higher to that of four B. cereus strains associated with periodontitis in humans and previously reported as the closest known strains to B. anthracis. SNP in ITS containing tRNA genes combined with RSI-PCR provide a very efficient tool for the identification of strains closely related to B. anthracis.}, author = {Daffonchio, Daniele and Raddadi, Noura and Merabishvili, Maya and Cherif, Ameur and Carmagnola, Lorenzo and Brusetti, Lorenzo and Rizzi, Aurora and Chanishvili, Nina and Visca, Paolo and Sharp, Richard and Borin, Sara}, citeulike-article-id = {2089932}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/AEM.72.2.1295-1301.2006}, citeulike-linkout-1 = {http://aem.asm.org/cgi/content/abstract/72/2/1295}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16461679}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16461679}, day = {1}, doi = {10.1128/AEM.72.2.1295-1301.2006}, issn = {0099-2240}, journal = {Applied and environmental microbiology}, keywords = {bacillus, evolution}, month = {February}, number = {2}, pages = {1295--1301}, posted-at = {2009-09-29 15:24:30}, priority = {2}, title = {Strategy for identification of Bacillus cereus and Bacillus thuringiensis strains closely related to Bacillus anthracis.}, url = {http://dx.doi.org/10.1128/AEM.72.2.1295-1301.2006}, volume = {72}, year = {2006} }

TY - JOUR ID - citeulike:2089932 L3 - citeulike-article-id:2089932 N2 - Bacillus cereus strains that are genetically closely related to B. anthracis can display anthrax-like virulence traits (A. R. Hoffmaster et al., Proc. Natl. Acad. Sci. USA 101:8449-8454, 2004). Hence, approaches that rapidly identify these "near neighbors" are of great interest for the study of B. anthracis virulence mechanisms, as well as to prevent the use of such strains for B. anthracis-based bioweapon development. Here, a strategy is proposed for the identification of near neighbors of B. anthracis based on single nucleotide polymorphisms (SNP) in the 16S-23S rRNA intergenic spacer (ITS) containing tRNA genes, characteristic of B. anthracis. By using restriction site insertion-PCR (RSI-PCR) the presence of two SNP typical of B. anthracis was screened in 126 B. cereus group strains of different origin. Two B. cereus strains and one B. thuringiensis strain showed RSI-PCR profiles identical to that of B. anthracis. The sequencing of the entire ITS containing tRNA genes revealed two of the strains to be identical to B. anthracis. The strict relationship with B. anthracis was confirmed by multilocus sequence typing (MLST) of four other independent loci: cerA, plcR, AC-390, and SG-749. The relationship to B. anthracis of the three strains described by MLST was comparable and even higher to that of four B. cereus strains associated with periodontitis in humans and previously reported as the closest known strains to B. anthracis. SNP in ITS containing tRNA genes combined with RSI-PCR provide a very efficient tool for the identification of strains closely related to B. anthracis. IS - 2 JF - Applied and environmental microbiology SN - 0099-2240 EP - 1301 TI - Strategy for identification of Bacillus cereus and Bacillus thuringiensis strains closely related to Bacillus anthracis. VL - 72 SP - 1295 KW - bacillus KW - evolution AU - Daffonchio, Daniele AU - Raddadi, Noura AU - Merabishvili, Maya AU - Cherif, Ameur AU - Carmagnola, Lorenzo AU - Brusetti, Lorenzo AU - Rizzi, Aurora AU - Chanishvili, Nina AU - Visca, Paolo AU - Sharp, Richard AU - Borin, Sara PY - 2006/02/01/ UR - http://dx.doi.org/10.1128/AEM.72.2.1295-1301.2006 ER -