MicroRazerS: Rapid alignment of small RNA reads Export

@article{citeulike:6040963, abstract = {Motivation: Deep sequencing has become the method of choice for determining the small RNA content of a cell. Mapping the sequenced reads onto their reference genome serves as the basis for all further analyses, namely for identification and quantification. A method frequently used is Mega BLAST followed by several filtering steps, even though it is slow and inefficient for this task. Also, none of the currently available short read aligners has established itself for the particular task of small RNA mapping. Results: We present MicroRazerS, a tool optimized for mapping small RNAs onto a reference genome. It is an order of magnitude faster than Mega BLAST and comparable in speed to other short read mapping tools. In addition, it is more sensitive and easy to handle and adjust. Availability: MicroRazerS is part of the SeqAn C++ library and can be downloaded from http://www.seqan.de/projects/MicroRazerS.html. Contact: emde@inf.fu-berlin.de, grunert@molgen.mpg.de 10.1093/bioinformatics/btp601}, author = {Emde, Anne-Katrin and Grunert, Marcel and Weese, David and Reinert, Knut and Sperling, Silke R.}, citeulike-article-id = {6040963}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/bioinformatics/btp601}, citeulike-linkout-1 = {http://bioinformatics.oxfordjournals.org/content/btp601v1/.abstract}, citeulike-linkout-2 = {http://bioinformatics.oxfordjournals.org/content/btp601v1/.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19880369}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19880369}, day = {29}, doi = {10.1093/bioinformatics/btp601}, journal = {Bioinformatics}, keywords = {alignment, next\_generation, rna-seq}, month = {October}, pages = {btp601+}, posted-at = {2009-11-02 08:11:59}, priority = {2}, title = {MicroRazerS: Rapid alignment of small RNA reads}, url = {http://dx.doi.org/10.1093/bioinformatics/btp601}, year = {2009} }

TY - JOUR ID - citeulike:6040963 L3 - citeulike-article-id:6040963 N2 - Motivation: Deep sequencing has become the method of choice for determining the small RNA content of a cell. Mapping the sequenced reads onto their reference genome serves as the basis for all further analyses, namely for identification and quantification. A method frequently used is Mega BLAST followed by several filtering steps, even though it is slow and inefficient for this task. Also, none of the currently available short read aligners has established itself for the particular task of small RNA mapping. Results: We present MicroRazerS, a tool optimized for mapping small RNAs onto a reference genome. It is an order of magnitude faster than Mega BLAST and comparable in speed to other short read mapping tools. In addition, it is more sensitive and easy to handle and adjust. Availability: MicroRazerS is part of the SeqAn C++ library and can be downloaded from http://www.seqan.de/projects/MicroRazerS.html. Contact: emde@inf.fu-berlin.de, grunert@molgen.mpg.de 10.1093/bioinformatics/btp601 JF - Bioinformatics TI - MicroRazerS: Rapid alignment of small RNA reads SP - btp601 KW - alignment KW - next_generation KW - rna-seq AU - Emde, Anne-Katrin AU - Grunert, Marcel AU - Weese, David AU - Reinert, Knut AU - Sperling, Silke PY - 2009/10/29/ UR - http://dx.doi.org/10.1093/bioinformatics/btp601 ER -