Endogenous blockage and delay of the chromosome cycle despite normal recruitment and growth phase explain poor proliferation and frequent edomitosis in Fanconi anemia cells. Export

@article{citeulike:1396637, abstract = {BrdU-Hoechst flow cytometry was employed to study the proliferation kinetics of blood lymphocytes from patients with Fanconi anemia (FA). Compared to controls, untreated FA lymphocytes show normal response to PHA stimulation, normal G0/G1 exit rates, and normal first S-phase durations. The G2 phase of the first cell cycle, however, is severely prolonged, and 24\% of the recruited population become arrested during the first chromosome cycle (S, G2/M phases). The delay suffered during G2 appears to be compensated in part by a subsequent G1 phase duration that is unusually short for postnatal human cells (3.7 +/- 0.5 hrs). In analogy to what has been observed in other cell systems after experimental delays of the chromosome cycle, we therefore postulate that at least some FA cells enter their second growth phase without prior completion of the delayed chromosome cycle. Renewed replication would ensue in such cells without prior passing through mitosis and cytokinesis, leading to endoreduplication, which is a frequent finding in the FA syndrome.}, author = {Kubbies, M. and Schindler, D. and Hoehn, H. and Schinzel, A. and Rabinovitch, P. S.}, citeulike-article-id = {1396637}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/4050789}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=4050789}, issn = {0002-9297}, journal = {Am J Hum Genet}, keywords = {arrest, cell, cycle, fanc}, month = {September}, number = {5}, pages = {1022--1030}, posted-at = {2007-06-18 12:04:51}, priority = {2}, title = {Endogenous blockage and delay of the chromosome cycle despite normal recruitment and growth phase explain poor proliferation and frequent edomitosis in Fanconi anemia cells.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/4050789}, volume = {37}, year = {1985} }

TY - JOUR ID - citeulike:1396637 L3 - citeulike-article-id:1396637 N2 - BrdU-Hoechst flow cytometry was employed to study the proliferation kinetics of blood lymphocytes from patients with Fanconi anemia (FA). Compared to controls, untreated FA lymphocytes show normal response to PHA stimulation, normal G0/G1 exit rates, and normal first S-phase durations. The G2 phase of the first cell cycle, however, is severely prolonged, and 24% of the recruited population become arrested during the first chromosome cycle (S, G2/M phases). The delay suffered during G2 appears to be compensated in part by a subsequent G1 phase duration that is unusually short for postnatal human cells (3.7 +/- 0.5 hrs). In analogy to what has been observed in other cell systems after experimental delays of the chromosome cycle, we therefore postulate that at least some FA cells enter their second growth phase without prior completion of the delayed chromosome cycle. Renewed replication would ensue in such cells without prior passing through mitosis and cytokinesis, leading to endoreduplication, which is a frequent finding in the FA syndrome. IS - 5 JF - Am J Hum Genet SN - 0002-9297 EP - 1030 TI - Endogenous blockage and delay of the chromosome cycle despite normal recruitment and growth phase explain poor proliferation and frequent edomitosis in Fanconi anemia cells. VL - 37 SP - 1022 KW - arrest KW - cell KW - cycle KW - fanc AU - Kubbies, M AU - Schindler, D AU - Hoehn, H AU - Schinzel, A AU - Rabinovitch, PS PY - 1985/09// UR - http://view.ncbi.nlm.nih.gov/pubmed/4050789 ER -