8-Methoxypsoralen plus 365 nm light effects and repair in yeast. Export

@article{citeulike:1877497, abstract = {Haploid wild-type and mutant cells of Saccharomyces carrying one of the single genes rad2-20 or rad9-4 and the double mutant rad2-20rad9-4 were tested for their response to a treatment with 8-methoxypsoralen plus 365 nm light using immediate and delayed plating techniques. The mutant defective in the excision of ultraviolet-induced pyrimidine dimers (rad2-20) as well as that presumably deficient in a recombinational repair system (rad9-4) are more sensitive than wild type cells. The double mutant (rad2-20rad9-4) demonstrates a higher sensitivity than each of the single mutants, indicating that at least two pathways are involved in the repair of the 8-methoxypsoralen plus 365 nm induced damages. In all cases survival curves have shoulders. The survival of wild type and rad9-4 cells is increased after dark holding whereas it remains constant for the rad2-20 mutant and for the double mutant. These results show that the induced damages are reparable. Respiratory deficient mutant (p-) were compared to the corresponding respiratory competent cells. It is shown that the respiratory function is required for the expression of the excision repair activity. The 8-methoxypsoralen plus 365 nm ligh treatment appears to be less effective than ultraviolet irradiation (254 nm) in the induction of the cytoplasmic 'petite' mutation at the same survival levels.}, author = {Averbeck, D. and Moustacchi, E.}, citeulike-article-id = {1877497}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/1148244}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=1148244}, day = {23}, issn = {0006-3002}, journal = {Biochim Biophys Acta}, keywords = {icl, incision, yeast}, month = {July}, number = {4}, pages = {393--404}, posted-at = {2007-11-07 12:11:24}, priority = {2}, title = {8-Methoxypsoralen plus 365 nm light effects and repair in yeast.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1148244}, volume = {395}, year = {1975} }

TY - JOUR ID - citeulike:1877497 L3 - citeulike-article-id:1877497 N2 - Haploid wild-type and mutant cells of Saccharomyces carrying one of the single genes rad2-20 or rad9-4 and the double mutant rad2-20rad9-4 were tested for their response to a treatment with 8-methoxypsoralen plus 365 nm light using immediate and delayed plating techniques. The mutant defective in the excision of ultraviolet-induced pyrimidine dimers (rad2-20) as well as that presumably deficient in a recombinational repair system (rad9-4) are more sensitive than wild type cells. The double mutant (rad2-20rad9-4) demonstrates a higher sensitivity than each of the single mutants, indicating that at least two pathways are involved in the repair of the 8-methoxypsoralen plus 365 nm induced damages. In all cases survival curves have shoulders. The survival of wild type and rad9-4 cells is increased after dark holding whereas it remains constant for the rad2-20 mutant and for the double mutant. These results show that the induced damages are reparable. Respiratory deficient mutant (p-) were compared to the corresponding respiratory competent cells. It is shown that the respiratory function is required for the expression of the excision repair activity. The 8-methoxypsoralen plus 365 nm ligh treatment appears to be less effective than ultraviolet irradiation (254 nm) in the induction of the cytoplasmic 'petite' mutation at the same survival levels. IS - 4 JF - Biochim Biophys Acta SN - 0006-3002 EP - 404 TI - 8-Methoxypsoralen plus 365 nm light effects and repair in yeast. VL - 395 SP - 393 KW - icl KW - incision KW - yeast AU - Averbeck, D AU - Moustacchi, E PY - 1975/07/23/ UR - http://view.ncbi.nlm.nih.gov/pubmed/1148244 ER -