Single-Gene Detection and Karyotyping Using Small-Target Fluorescence in Situ Hybridization on Maize Somatic Chromosomes Export

@article{citeulike:1780390, abstract = {Combined with a system for identifying each of the chromosomes in a genome, visualizing the location of individual genetic loci by fluorescence in situ hybridization (FISH) would aid in assembling physical and genetic maps. Previously, large genomic clones have been successfully used as FISH probes onto somatic chromosomes but this approach is complicated in species with abundant repetitive elements. In this study, repeat-free portions of sequences that were anchored to particular chromosomes including genes, gene clusters, large cDNAs, and portions of BACs obtained from public databases were used to label the corresponding physical location using FISH. A collection of probes that includes at least one marker on each chromosome in the maize complement was assembled, allowing a small-target karyotyping system to be developed. This set provides the foundation onto which additional loci could be added to strengthen further the ability to perform chromosomal identification in maize and its relatives. The probes were demonstrated to produce signals in several wild relatives of maize, including Zea luxurians, Z. diploperennis, and Tripsacum dactyloides. 10.1534/genetics.106.065573}, author = {Lamb, Jonathan C. and Danilova, Tatiana and Bauer, Matthew J. and Meyer, Julie M. and Holland, Jennifer J. and Jensen, Michael D. and Birchler, James A.}, citeulike-article-id = {1780390}, citeulike-linkout-0 = {http://dx.doi.org/10.1534/genetics.106.065573}, citeulike-linkout-1 = {http://www.genetics.org/cgi/content/abstract/175/3/1047}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17237520}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17237520}, day = {1}, doi = {10.1534/genetics.106.065573}, journal = {Genetics}, keywords = {fish, methods}, month = {March}, number = {3}, pages = {1047--1058}, posted-at = {2007-10-17 17:01:54}, priority = {2}, title = {Single-Gene Detection and Karyotyping Using Small-Target Fluorescence in Situ Hybridization on Maize Somatic Chromosomes}, url = {http://dx.doi.org/10.1534/genetics.106.065573}, volume = {175}, year = {2007} }

TY - JOUR ID - citeulike:1780390 L3 - citeulike-article-id:1780390 N2 - Combined with a system for identifying each of the chromosomes in a genome, visualizing the location of individual genetic loci by fluorescence in situ hybridization (FISH) would aid in assembling physical and genetic maps. Previously, large genomic clones have been successfully used as FISH probes onto somatic chromosomes but this approach is complicated in species with abundant repetitive elements. In this study, repeat-free portions of sequences that were anchored to particular chromosomes including genes, gene clusters, large cDNAs, and portions of BACs obtained from public databases were used to label the corresponding physical location using FISH. A collection of probes that includes at least one marker on each chromosome in the maize complement was assembled, allowing a small-target karyotyping system to be developed. This set provides the foundation onto which additional loci could be added to strengthen further the ability to perform chromosomal identification in maize and its relatives. The probes were demonstrated to produce signals in several wild relatives of maize, including Zea luxurians, Z. diploperennis, and Tripsacum dactyloides. 10.1534/genetics.106.065573 IS - 3 JF - Genetics EP - 1058 TI - Single-Gene Detection and Karyotyping Using Small-Target Fluorescence in Situ Hybridization on Maize Somatic Chromosomes VL - 175 SP - 1047 KW - fish KW - methods AU - Lamb, Jonathan AU - Danilova, Tatiana AU - Bauer, Matthew AU - Meyer, Julie AU - Holland, Jennifer AU - Jensen, Michael AU - Birchler, James PY - 2007/03/01/ UR - http://dx.doi.org/10.1534/genetics.106.065573 ER -