Role of Petal-Specific Orcinol O-Methyltransferases in the Evolution of Rose Scent Export

@article{citeulike:2706572, abstract = {Orcinol O-methyltransferase (OOMT) 1 and 2 catalyze the last two steps of the biosynthetic pathway leading to the phenolic methyl ether 3,5-dimethoxytoluene (DMT), the major scent compound of many rose (Rosa x hybrida) varieties. Modern roses are descended from both European and Chinese species, the latter being producers of phenolic methyl ethers but not the former. Here we investigated why phenolic methyl ether production occurs in some but not all rose varieties. In DMT-producing varieties, OOMTs were shown to be localized specifically in the petal, predominanty in the adaxial epidermal cells. In these cells, OOMTs become increasingly associated with membranes during petal development, suggesting that the scent biosynthesis pathway catalyzed by these enzymes may be directly linked to the cells' secretory machinery. OOMT gene sequences were detected in two non-DMT-producing rose species of European origin, but no mRNA transcripts were detected, and these varieties lacked both OOMT protein and enzyme activity. These data indicate that up-regulation of OOMT gene expression may have been a critical step in the evolution of scent production in roses. 10.1104/pp.105.070961}, author = {Scalliet, Gabriel and Lionnet, Claire and Le Bechec, Mickael and Dutron, Laurence and Magnard, Jean-Louis and Baudino, Sylvie and Bergougnoux, Veronique and Jullien, Frederic and Chambrier, Pierre and Vergne, Philippe and Dumas, Christian and Cock, Mark J. and Hugueney, Philippe}, citeulike-article-id = {2706572}, citeulike-linkout-0 = {http://dx.doi.org/10.1104/pp.105.070961}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16361520}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16361520}, day = {1}, doi = {10.1104/pp.105.070961}, journal = {Plant Physiol.}, keywords = {evolution, general\_interest}, month = {January}, number = {1}, pages = {18--29}, posted-at = {2008-04-23 09:48:31}, priority = {2}, title = {Role of Petal-Specific Orcinol O-Methyltransferases in the Evolution of Rose Scent}, url = {http://dx.doi.org/10.1104/pp.105.070961}, volume = {140}, year = {2006} }

TY - JOUR ID - citeulike:2706572 L3 - citeulike-article-id:2706572 N2 - Orcinol O-methyltransferase (OOMT) 1 and 2 catalyze the last two steps of the biosynthetic pathway leading to the phenolic methyl ether 3,5-dimethoxytoluene (DMT), the major scent compound of many rose (Rosa x hybrida) varieties. Modern roses are descended from both European and Chinese species, the latter being producers of phenolic methyl ethers but not the former. Here we investigated why phenolic methyl ether production occurs in some but not all rose varieties. In DMT-producing varieties, OOMTs were shown to be localized specifically in the petal, predominanty in the adaxial epidermal cells. In these cells, OOMTs become increasingly associated with membranes during petal development, suggesting that the scent biosynthesis pathway catalyzed by these enzymes may be directly linked to the cells' secretory machinery. OOMT gene sequences were detected in two non-DMT-producing rose species of European origin, but no mRNA transcripts were detected, and these varieties lacked both OOMT protein and enzyme activity. These data indicate that up-regulation of OOMT gene expression may have been a critical step in the evolution of scent production in roses. 10.1104/pp.105.070961 IS - 1 JF - Plant Physiol. EP - 29 TI - Role of Petal-Specific Orcinol O-Methyltransferases in the Evolution of Rose Scent VL - 140 SP - 18 KW - evolution KW - general_interest AU - Scalliet, Gabriel AU - Lionnet, Claire AU - Le Bechec, Mickael AU - Dutron, Laurence AU - Magnard, Jean-Louis AU - Baudino, Sylvie AU - Bergougnoux, Veronique AU - Jullien, Frederic AU - Chambrier, Pierre AU - Vergne, Philippe AU - Dumas, Christian AU - Cock, Mark AU - Hugueney, Philippe PY - 2006/01/01/ UR - http://dx.doi.org/10.1104/pp.105.070961 ER -