Using real-time PCR to determine transgene copy number in wheat Export

@article{citeulike:2789272, abstract = {Abstract\ \ Transgene copy number is usually determined by means of Southern blot analysis which can be time consuming and laborious. In this study, quantitative real-time PCR was developed to determine transgene copy number in transgenic wheat. A conserved wheat housekeeping gene,puroindoline-b, was used as an internal control to calculate transgene copy number. Estimated copy number in transgenic lines using real-time quantitative PCR was correlated with actual copy number based on Southern blot analysis. Real-time PCR can analyze hundreds of samples in a day, making it an efficient method for estimating copy number in transgenic wheat.}, author = {Li, Zhiwu and Hansen, Jennifer and Liu, Ying and Zemetra, Robert and Berger, Philip}, citeulike-article-id = {2789272}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/BF02772725}, day = {15}, doi = {10.1007/BF02772725}, journal = {Plant Molecular Biology Reporter}, keywords = {gene\_copy\_number, methods}, month = {June}, number = {2}, pages = {179--188}, posted-at = {2008-05-12 12:36:17}, priority = {2}, title = {Using real-time PCR to determine transgene copy number in wheat}, url = {http://dx.doi.org/10.1007/BF02772725}, volume = {22}, year = {2004} }

TY - JOUR ID - citeulike:2789272 L3 - citeulike-article-id:2789272 N2 - Abstract  Transgene copy number is usually determined by means of Southern blot analysis which can be time consuming and laborious. In this study, quantitative real-time PCR was developed to determine transgene copy number in transgenic wheat. A conserved wheat housekeeping gene,puroindoline-b, was used as an internal control to calculate transgene copy number. Estimated copy number in transgenic lines using real-time quantitative PCR was correlated with actual copy number based on Southern blot analysis. Real-time PCR can analyze hundreds of samples in a day, making it an efficient method for estimating copy number in transgenic wheat. IS - 2 JF - Plant Molecular Biology Reporter EP - 188 TI - Using real-time PCR to determine transgene copy number in wheat VL - 22 SP - 179 KW - gene_copy_number KW - methods AU - Li, Zhiwu AU - Hansen, Jennifer AU - Liu, Ying AU - Zemetra, Robert AU - Berger, Philip PY - 2004/06/15/ UR - http://dx.doi.org/10.1007/BF02772725 ER -