DNA ploidy levels and intraspecific DNA content variability in Romanian fescues ( Festuca, Poaceae ) measured in fresh and herbarium material Export

@article{citeulike:3091067, abstract = {Abstract\ \ DNA ploidy level estimates are presented for 11 species and two natural hybrids ofFestuca sampled from 39 locations in Romania. Altogether 48 living samples (22 of them also as one-year-old herbarium specimens) and additional 65 one-year-old herbarium specimens were analyzed using flow cytometry with DAPI staining. The following DNA ploidy levels were assessed:F. callieri (4x),F. ovina (2x),F. pallens (2x),F. polesica (2x),F. pseudodalmatica (2x, 4x, 5x),F. pseudovaginata (2x),F. pseudovina (2x),F. pseudovina ×F. rupicola (4x),F. rupicola (6x),F. vaginata (2x),F. vaginata ×F. valesiaca (2x),F. valesiaca (2x) andF. xanthina (2x).Festuca pseudovaginata is reported for the flora of Romania for the first time. Measurements of one-year-old herbarium specimens produced significantly smaller sample/standard ratios (P\<0.001) and higher coefficients of variance (P \< 0.001) when compared with fresh plant samples. Several species exhibited marked intraspecific nuclear DNA content variability, documented by non-overlapping double-peaks or bimodal peaks in simultaneous measurements. A maximum difference of about 9.2\% in relative DNA contents was observed inF. pallens, 5.5\% inF. polesica, 4.2\% inF. vaginata, and 3.8\% inF. rupicola. DiploidF. xanhina (Festuca sect.Eskia) had 1.39–1.58 times higher monoploid relative DNA content in relation to the species of sectionFestuca.}, author = {\v{S}marda, Petr}, citeulike-article-id = {3091067}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/BF02806558}, day = {11}, doi = {10.1007/BF02806558}, journal = {Folia Geobotanica}, keywords = {flow\_cytometry, polyploidy}, month = {December}, number = {4}, pages = {417--432}, posted-at = {2008-08-06 16:52:59}, priority = {2}, title = {DNA ploidy levels and intraspecific DNA content variability in Romanian fescues ( Festuca, Poaceae ) measured in fresh and herbarium material}, url = {http://dx.doi.org/10.1007/BF02806558}, volume = {41}, year = {2006} }

TY - JOUR ID - citeulike:3091067 L3 - citeulike-article-id:3091067 N2 - Abstract  DNA ploidy level estimates are presented for 11 species and two natural hybrids ofFestuca sampled from 39 locations in Romania. Altogether 48 living samples (22 of them also as one-year-old herbarium specimens) and additional 65 one-year-old herbarium specimens were analyzed using flow cytometry with DAPI staining. The following DNA ploidy levels were assessed:F. callieri (4x),F. ovina (2x),F. pallens (2x),F. polesica (2x),F. pseudodalmatica (2x, 4x, 5x),F. pseudovaginata (2x),F. pseudovina (2x),F. pseudovina ×F. rupicola (4x),F. rupicola (6x),F. vaginata (2x),F. vaginata ×F. valesiaca (2x),F. valesiaca (2x) andF. xanthina (2x).Festuca pseudovaginata is reported for the flora of Romania for the first time. Measurements of one-year-old herbarium specimens produced significantly smaller sample/standard ratios (P<0.001) and higher coefficients of variance (P < 0.001) when compared with fresh plant samples. Several species exhibited marked intraspecific nuclear DNA content variability, documented by non-overlapping double-peaks or bimodal peaks in simultaneous measurements. A maximum difference of about 9.2% in relative DNA contents was observed inF. pallens, 5.5% inF. polesica, 4.2% inF. vaginata, and 3.8% inF. rupicola. DiploidF. xanhina (Festuca sect.Eskia) had 1.39–1.58 times higher monoploid relative DNA content in relation to the species of sectionFestuca. IS - 4 JF - Folia Geobotanica EP - 432 TI - DNA ploidy levels and intraspecific DNA content variability in Romanian fescues ( Festuca, Poaceae ) measured in fresh and herbarium material VL - 41 SP - 417 KW - flow_cytometry KW - polyploidy AU - Šmarda, Petr PY - 2006/12/11/ UR - http://dx.doi.org/10.1007/BF02806558 ER -