Differential expression of candidate salt-tolerance genes in the halophyte Helianthus paradoxus and its glycophyte progenitors H. annuus and H. petiolaris (Asteraceae) Export

@article{citeulike:5984228, abstract = {Adaptation to different habitats is considered to be a major force in the generation of organismal diversity. Understanding the genetic mechanisms that produce such adaptations will provide insights into long-standing questions in evolutionary biology and, at the same time, improve predictions of plant responses to changing environmental conditions. Here we used semiquantitative RT-PCR to study the expression of eight candidate salt-tolerance genes in leaves of the highly salt-tolerant diploid hybrid species Helianthus paradoxus and its salt-sensitive progenitor species H. annuus and H. petiolaris. Samples were collected after germination and growth under four different treatments: nonsaline (control), near-natural saline, saline with increased K+, and saline with decreased Mg2+ and Ca2+. Three individuals from three populations per species were used. The hybrid species H. paradoxus constitutively under- or overexpressed genes related to potassium and calcium transport (homologues of KT1, KT2, ECA1), suggesting that these genes may contribute to the adaptation of H. paradoxus to salinity. In two other genes, variation between populations within species exceeded species level variation. Furthermore, homologues of the potassium transporter HAK8 and of a transcriptional regulator were generally overexpressed in saline treatments, suggesting that these genes are involved in sustained growth under saline conditions in Helianthus. 10.3732/ajb.0900067}, author = {Edelist, Cecile and Raffoux, Xavier and Falque, Matthieu and Dillmann, Christine and Sicard, Delphine and Rieseberg, Loren H. and Karrenberg, Sophie}, citeulike-article-id = {5984228}, citeulike-linkout-0 = {http://dx.doi.org/10.3732/ajb.0900067}, citeulike-linkout-1 = {http://www.amjbot.org/content/96/10/1830.abstract}, citeulike-linkout-2 = {http://www.amjbot.org/content/96/10/1830.full.pdf}, day = {1}, doi = {10.3732/ajb.0900067}, journal = {Am. J. Bot.}, keywords = {gene\_expression, hybridization}, month = {October}, number = {10}, pages = {1830--1838}, posted-at = {2009-10-22 09:51:03}, priority = {2}, title = {Differential expression of candidate salt-tolerance genes in the halophyte Helianthus paradoxus and its glycophyte progenitors H. annuus and H. petiolaris (Asteraceae)}, url = {http://dx.doi.org/10.3732/ajb.0900067}, volume = {96}, year = {2009} }

TY - JOUR ID - citeulike:5984228 L3 - citeulike-article-id:5984228 N2 - Adaptation to different habitats is considered to be a major force in the generation of organismal diversity. Understanding the genetic mechanisms that produce such adaptations will provide insights into long-standing questions in evolutionary biology and, at the same time, improve predictions of plant responses to changing environmental conditions. Here we used semiquantitative RT-PCR to study the expression of eight candidate salt-tolerance genes in leaves of the highly salt-tolerant diploid hybrid species Helianthus paradoxus and its salt-sensitive progenitor species H. annuus and H. petiolaris. Samples were collected after germination and growth under four different treatments: nonsaline (control), near-natural saline, saline with increased K+, and saline with decreased Mg2+ and Ca2+. Three individuals from three populations per species were used. The hybrid species H. paradoxus constitutively under- or overexpressed genes related to potassium and calcium transport (homologues of KT1, KT2, ECA1), suggesting that these genes may contribute to the adaptation of H. paradoxus to salinity. In two other genes, variation between populations within species exceeded species level variation. Furthermore, homologues of the potassium transporter HAK8 and of a transcriptional regulator were generally overexpressed in saline treatments, suggesting that these genes are involved in sustained growth under saline conditions in Helianthus. 10.3732/ajb.0900067 IS - 10 JF - Am. J. Bot. EP - 1838 TI - Differential expression of candidate salt-tolerance genes in the halophyte Helianthus paradoxus and its glycophyte progenitors H. annuus and H. petiolaris (Asteraceae) VL - 96 SP - 1830 KW - gene_expression KW - hybridization AU - Edelist, Cecile AU - Raffoux, Xavier AU - Falque, Matthieu AU - Dillmann, Christine AU - Sicard, Delphine AU - Rieseberg, Loren AU - Karrenberg, Sophie PY - 2009/10/01/ UR - http://dx.doi.org/10.3732/ajb.0900067 ER -