Bright far-red fluorescent protein for whole-body imaging. Export

@article{citeulike:1604828, abstract = {For deep imaging of animal tissues, the optical window favorable for light penetration is in near-infrared wavelengths, which requires proteins with emission spectra in the far-red wavelengths. Here we report a far-red fluorescent protein, named Katushka, which is seven- to tenfold brighter compared to the spectrally close HcRed or mPlum, and is characterized by fast maturation as well as a high pH-stability and photostability. These unique characteristics make Katushka the protein of choice for visualization in living tissues. We demonstrate superiority of Katushka for whole-body imaging by direct comparison with other red and far-red fluorescent proteins. We also describe a monomeric version of Katushka, named mKate, which is characterized by high brightness and photostability, and should be an excellent fluorescent label for protein tagging in the far-red part of the spectrum.}, address = {[1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia. [2] These authors contributed equally to this work.}, author = {Shcherbo, Dmitry and Merzlyak, Ekaterina M. and Chepurnykh, Tatiana V. and Fradkov, Arkady F. and Ermakova, Galina V. and Solovieva, Elena A. and Lukyanov, Konstantin A. and Bogdanova, Ekaterina A. and Zaraisky, Andrey G. and Lukyanov, Sergey and Chudakov, Dmitriy M.}, citeulike-article-id = {1604828}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nmeth1083}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nmeth1083}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17721542}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17721542}, day = {26}, doi = {10.1038/nmeth1083}, issn = {1548-7091}, journal = {Nature methods}, keywords = {fluorescence, gfp, protein}, month = {September}, number = {9}, pages = {741--746}, posted-at = {2008-02-05 21:47:46}, priority = {2}, title = {Bright far-red fluorescent protein for whole-body imaging.}, url = {http://dx.doi.org/10.1038/nmeth1083}, volume = {4}, year = {2007} }

TY - JOUR ID - citeulike:1604828 L3 - citeulike-article-id:1604828 N2 - For deep imaging of animal tissues, the optical window favorable for light penetration is in near-infrared wavelengths, which requires proteins with emission spectra in the far-red wavelengths. Here we report a far-red fluorescent protein, named Katushka, which is seven- to tenfold brighter compared to the spectrally close HcRed or mPlum, and is characterized by fast maturation as well as a high pH-stability and photostability. These unique characteristics make Katushka the protein of choice for visualization in living tissues. We demonstrate superiority of Katushka for whole-body imaging by direct comparison with other red and far-red fluorescent proteins. We also describe a monomeric version of Katushka, named mKate, which is characterized by high brightness and photostability, and should be an excellent fluorescent label for protein tagging in the far-red part of the spectrum. IS - 9 JF - Nature methods SN - 1548-7091 AD - [1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia. [2] These authors contributed equally to this work. EP - 746 TI - Bright far-red fluorescent protein for whole-body imaging. VL - 4 SP - 741 KW - fluorescence KW - gfp KW - protein AU - Shcherbo, Dmitry AU - Merzlyak, Ekaterina AU - Chepurnykh, Tatiana AU - Fradkov, Arkady AU - Ermakova, Galina AU - Solovieva, Elena AU - Lukyanov, Konstantin AU - Bogdanova, Ekaterina AU - Zaraisky, Andrey AU - Lukyanov, Sergey AU - Chudakov, Dmitriy PY - 2007/09/26/ UR - http://dx.doi.org/10.1038/nmeth1083 ER -