Rejection of two-photon fluorescence background in thick tissue bydifferential aberration imaging Export

@article{citeulike:2662662, abstract = {We present a simple and robust way to reject out-of-focus background when performing deep two-photon excited fluorescence (TPEF) imaging in thick tissue. The technique is based on the use of a deformable mirror (DM) to introduce illumination aberrations that preferentially degrade TPEF signal while leaving TPEF background relatively unchanged. A subtraction of aberrated from unaberrated images leads to background rejection. We present a heuristic description of our technique, which we corroborate with experiment. An added benefit of our technique is that it leads to somewhat improved image resolution.}, author = {Leray, Aymeric and Mertz, Jerome}, citeulike-article-id = {2662662}, citeulike-linkout-0 = {http://www.opticsinfobase.org/abstract.cfm?id=116570}, day = {30}, journal = {Opt. Express}, keywords = {microscopy, shg}, month = {October}, number = {22}, pages = {10565--10573}, posted-at = {2008-04-13 03:19:06}, priority = {2}, publisher = {OSA}, title = {Rejection of two-photon fluorescence background in thick tissue bydifferential aberration imaging}, url = {http://www.opticsinfobase.org/abstract.cfm?id=116570}, volume = {14}, year = {2006} }

TY - JOUR ID - citeulike:2662662 L3 - citeulike-article-id:2662662 N2 - We present a simple and robust way to reject out-of-focus background when performing deep two-photon excited fluorescence (TPEF) imaging in thick tissue. The technique is based on the use of a deformable mirror (DM) to introduce illumination aberrations that preferentially degrade TPEF signal while leaving TPEF background relatively unchanged. A subtraction of aberrated from unaberrated images leads to background rejection. We present a heuristic description of our technique, which we corroborate with experiment. An added benefit of our technique is that it leads to somewhat improved image resolution. IS - 22 JF - Opt. Express EP - 10573 TI - Rejection of two-photon fluorescence background in thick tissue bydifferential aberration imaging PB - OSA VL - 14 SP - 10565 KW - microscopy KW - shg AU - Leray, Aymeric AU - Mertz, Jerome PY - 2006/10/30/ UR - http://www.opticsinfobase.org/abstract.cfm?id=116570 ER -