Control of the blue fluorescent protein with advanced evolutionary pulse shaping Export

@article{citeulike:3378199, abstract = {We demonstrate optical coherent control of the two-photon fluorescence of the blue fluorescent protein (BFP), which is of interest in investigations of protein–protein interactions. In addition to biological relevance, BFP represents an interesting target for coherent control from a chemical perspective due to its many components of highly nonexponential fluorescence decay and low quantum yield resulting from excited state isomerization. Using a genetic algorithm with a multiplicative (rather than ratiometric) fitness parameter, we are able to control the ratio of BFP fluorescence to second-harmonic generation without a considerable drop in the maximized signal. The importance of linear chirp and power-scaling on the discrimination process is investigated in detail.}, author = {Tkaczyk, E. and Mauring, K. and Tkaczyk, A. and Krasnenko, V. and Ye, J. and Bakerjr, J. and Norris, T.}, citeulike-article-id = {3378199}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.bbrc.2008.09.075}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0006291X08018238}, day = {28}, doi = {10.1016/j.bbrc.2008.09.075}, issn = {0006291X}, journal = {Biochemical and Biophysical Research Communications}, keywords = {bfp, mine}, month = {November}, number = {4}, pages = {733--737}, posted-at = {2008-10-21 17:55:14}, priority = {2}, title = {Control of the blue fluorescent protein with advanced evolutionary pulse shaping}, url = {http://dx.doi.org/10.1016/j.bbrc.2008.09.075}, volume = {376}, year = {2008} }

TY - JOUR ID - citeulike:3378199 L3 - citeulike-article-id:3378199 N2 - We demonstrate optical coherent control of the two-photon fluorescence of the blue fluorescent protein (BFP), which is of interest in investigations of protein–protein interactions. In addition to biological relevance, BFP represents an interesting target for coherent control from a chemical perspective due to its many components of highly nonexponential fluorescence decay and low quantum yield resulting from excited state isomerization. Using a genetic algorithm with a multiplicative (rather than ratiometric) fitness parameter, we are able to control the ratio of BFP fluorescence to second-harmonic generation without a considerable drop in the maximized signal. The importance of linear chirp and power-scaling on the discrimination process is investigated in detail. IS - 4 JF - Biochemical and Biophysical Research Communications SN - 0006291X EP - 737 TI - Control of the blue fluorescent protein with advanced evolutionary pulse shaping VL - 376 SP - 733 KW - bfp KW - mine AU - Tkaczyk, E AU - Mauring, K AU - Tkaczyk, A AU - Krasnenko, V AU - Ye, J AU - Bakerjr, J AU - Norris, T PY - 2008/11/28/ UR - http://dx.doi.org/10.1016/j.bbrc.2008.09.075 ER -