Identification of a naturally processed cyclin D1 T-helper epitope by a novel combination of HLA class II targeting and differential mass spectrometry. Export

@article{citeulike:2549252, abstract = {T-helper (Th) cells play an important role in orchestrating the effector function of CTL in anti-tumor immunity. However, only a limited number of Th cell epitopes has been characterized. Here we describe a novel approach for identifying naturally processed and presented peptides derived from chosen antigens. This method combines a transfection step of antigen-presenting cells with a vector encoding a fusion protein between the Ii chain and the antigen of interest, elution of the HLA-bound peptides and identification of the antigen-derived peptides by mass spectrometric comparison to the non-transfected cells. In vitro-stimulated Th cells against the identified peptide of interest specifically recognize transfectants overexpressing the cognate antigen. Using this approach, we were able to identify the HLA-DR4-restricted Th cell epitope NPPSMVAAGSVVAAV derived from cyclin D1, which is frequently overexpressed in tumors. This method will help in identifying peptide candidates for vaccination studies for tumor immunotherapy.}, address = {Department of Immunology, Institute for Cell Biology, University of T\"{u}bingen, T\"{u}bingen, Germany.}, author = {Dengjel, J. and Decker, P. and Schoor, O. and Altenberend, F. and Weinschenk, T. and Rammensee, H. G. and Stevanovic, S.}, citeulike-article-id = {2549252}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/eji.200425510}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15495271}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15495271}, doi = {10.1002/eji.200425510}, issn = {0014-2980}, journal = {Eur J Immunol}, keywords = {ccnd1, cd4}, month = {December}, number = {12}, pages = {3644--3651}, posted-at = {2008-03-18 08:14:33}, priority = {2}, title = {Identification of a naturally processed cyclin D1 T-helper epitope by a novel combination of HLA class II targeting and differential mass spectrometry.}, url = {http://dx.doi.org/10.1002/eji.200425510}, volume = {34}, year = {2004} }

TY - JOUR ID - citeulike:2549252 L3 - citeulike-article-id:2549252 N2 - T-helper (Th) cells play an important role in orchestrating the effector function of CTL in anti-tumor immunity. However, only a limited number of Th cell epitopes has been characterized. Here we describe a novel approach for identifying naturally processed and presented peptides derived from chosen antigens. This method combines a transfection step of antigen-presenting cells with a vector encoding a fusion protein between the Ii chain and the antigen of interest, elution of the HLA-bound peptides and identification of the antigen-derived peptides by mass spectrometric comparison to the non-transfected cells. In vitro-stimulated Th cells against the identified peptide of interest specifically recognize transfectants overexpressing the cognate antigen. Using this approach, we were able to identify the HLA-DR4-restricted Th cell epitope NPPSMVAAGSVVAAV derived from cyclin D1, which is frequently overexpressed in tumors. This method will help in identifying peptide candidates for vaccination studies for tumor immunotherapy. IS - 12 JF - Eur J Immunol SN - 0014-2980 AD - Department of Immunology, Institute for Cell Biology, University of Tübingen, Tübingen, Germany. EP - 3651 TI - Identification of a naturally processed cyclin D1 T-helper epitope by a novel combination of HLA class II targeting and differential mass spectrometry. VL - 34 SP - 3644 KW - ccnd1 KW - cd4 AU - Dengjel, J AU - Decker, P AU - Schoor, O AU - Altenberend, F AU - Weinschenk, T AU - Rammensee, HG AU - Stevanovic, S PY - 2004/12// UR - http://dx.doi.org/10.1002/eji.200425510 ER -