Prion infection of epithelial Rov cells is a polarized event. Export

@article{citeulike:3366436, abstract = {During prion infections, the cellular glycosylphosphatidylinositol-anchored glycoprotein PrP is converted into a conformational isoform. This abnormal conformer is thought to recruit and convert the normal cellular PrP into a likeness of itself and is proposed to be the infectious agent. We investigated the distribution of the PrP protein on the surface of Rov cells, an epithelial cell line highly permissive to prion multiplication, and we found that PrP is primarily expressed on the apical side. We further show that prion transmission to Rov cells is much more efficient if infectivity contacts the apical side, indicating that the apical and basolateral sides of Rov cells are not equally competent for prion infection and adding prions to the list of the conventional infectious agents (viruses and bacteria) that infect epithelial cells in a polarized manner. These data raise the possibility that apically expressed PrP may be involved in this polarized process of infection. This would add further support for a crucial role of PrP at the cell surface in prion infection of target cells.}, author = {Paquet, S. and Sabuncu, E. and Delaunay, J. L. and Laude, H. and Vilette, D.}, citeulike-article-id = {3366436}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JVI.78.13.7148-7152.2004}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15194791}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15194791}, doi = {10.1128/JVI.78.13.7148-7152.2004}, issn = {0022-538X}, journal = {Journal of virology}, keywords = {cellular\_model, cellular\_polarisation, prions}, month = {July}, number = {13}, pages = {7148--7152}, posted-at = {2008-10-02 14:49:13}, priority = {0}, title = {Prion infection of epithelial Rov cells is a polarized event.}, url = {http://dx.doi.org/10.1128/JVI.78.13.7148-7152.2004}, volume = {78}, year = {2004} }

TY - JOUR ID - citeulike:3366436 L3 - citeulike-article-id:3366436 N2 - During prion infections, the cellular glycosylphosphatidylinositol-anchored glycoprotein PrP is converted into a conformational isoform. This abnormal conformer is thought to recruit and convert the normal cellular PrP into a likeness of itself and is proposed to be the infectious agent. We investigated the distribution of the PrP protein on the surface of Rov cells, an epithelial cell line highly permissive to prion multiplication, and we found that PrP is primarily expressed on the apical side. We further show that prion transmission to Rov cells is much more efficient if infectivity contacts the apical side, indicating that the apical and basolateral sides of Rov cells are not equally competent for prion infection and adding prions to the list of the conventional infectious agents (viruses and bacteria) that infect epithelial cells in a polarized manner. These data raise the possibility that apically expressed PrP may be involved in this polarized process of infection. This would add further support for a crucial role of PrP at the cell surface in prion infection of target cells. IS - 13 JF - Journal of virology SN - 0022-538X EP - 7152 TI - Prion infection of epithelial Rov cells is a polarized event. VL - 78 SP - 7148 KW - cellular_model KW - cellular_polarisation KW - prions AU - Paquet, S AU - Sabuncu, E AU - Delaunay, JL AU - Laude, H AU - Vilette, D PY - 2004/07// UR - http://dx.doi.org/10.1128/JVI.78.13.7148-7152.2004 ER -