Identification of a novel mouse P4-ATPase family member highly expressed during spermatogenesis Export

@article{citeulike:5376961, abstract = {P4-ATPases are transmembrane proteins unique to eukaryotes that play a fundamental role in vesicular transport. They have been proposed to act as phospholipid flippases thereby regulating lipid topology in cellular membranes. We cloned and characterized a novel murine P4-ATPase that is specifically expressed in testis, and named it FetA (flippase expressed in testis splicing form A). When expressed in Saccharomyces cerevisiae, FetA localizes partially to the plasma membrane resulting in increased internalization of NBD-labeled phosphatidylethanolamine and phosphatidylcholine, supporting a role for FetA in the inward lipid translocation across cellular membranes. In mouse testis, FetA protein is detected in gamete cells, from pachytene spermatocytes to mature sperms, and its intracellular localization is tightly related with acrosome formation, a process that involves intensive intracellular vesicle formation and fusion. Furthermore, loss-of-function of FetA by RNA interference in mastocytoma P815 cells profoundly perturbs the structural organization of the Golgi complex and causes loss of constitutive secretion at lower temperature. Our findings point to an essential role of FetA in Golgi morphology and secretory function, suggesting a crucial role for this novel murine P4-ATPase in spermatogenesis. 10.1242/jcs.047423}, author = {Xu, Peng and Okkeri, Juha and Hanisch, Susanne and Hu, Rui-Ying and Xu, Qin and Pomorski, Thomas G. and Ding, Xiao-Yan}, citeulike-article-id = {5376961}, citeulike-linkout-0 = {http://dx.doi.org/10.1242/jcs.047423}, citeulike-linkout-1 = {http://jcs.biologists.org/cgi/content/abstract/122/16/2866}, day = {15}, doi = {10.1242/jcs.047423}, journal = {J Cell Sci}, keywords = {feta}, month = {August}, number = {16}, pages = {2866--2876}, posted-at = {2009-08-06 06:52:54}, priority = {2}, title = {Identification of a novel mouse P4-ATPase family member highly expressed during spermatogenesis}, url = {http://dx.doi.org/10.1242/jcs.047423}, volume = {122}, year = {2009} }

TY - JOUR ID - citeulike:5376961 L3 - citeulike-article-id:5376961 N2 - P4-ATPases are transmembrane proteins unique to eukaryotes that play a fundamental role in vesicular transport. They have been proposed to act as phospholipid flippases thereby regulating lipid topology in cellular membranes. We cloned and characterized a novel murine P4-ATPase that is specifically expressed in testis, and named it FetA (flippase expressed in testis splicing form A). When expressed in Saccharomyces cerevisiae, FetA localizes partially to the plasma membrane resulting in increased internalization of NBD-labeled phosphatidylethanolamine and phosphatidylcholine, supporting a role for FetA in the inward lipid translocation across cellular membranes. In mouse testis, FetA protein is detected in gamete cells, from pachytene spermatocytes to mature sperms, and its intracellular localization is tightly related with acrosome formation, a process that involves intensive intracellular vesicle formation and fusion. Furthermore, loss-of-function of FetA by RNA interference in mastocytoma P815 cells profoundly perturbs the structural organization of the Golgi complex and causes loss of constitutive secretion at lower temperature. Our findings point to an essential role of FetA in Golgi morphology and secretory function, suggesting a crucial role for this novel murine P4-ATPase in spermatogenesis. 10.1242/jcs.047423 IS - 16 JF - J Cell Sci EP - 2876 TI - Identification of a novel mouse P4-ATPase family member highly expressed during spermatogenesis VL - 122 SP - 2866 KW - feta AU - Xu, Peng AU - Okkeri, Juha AU - Hanisch, Susanne AU - Hu, Rui-Ying AU - Xu, Qin AU - Pomorski, Thomas AU - Ding, Xiao-Yan PY - 2009/08/15/ UR - http://dx.doi.org/10.1242/jcs.047423 ER -