Histone deacetylase inhibitor-induced glycodelin enhances the initial step of implantation Export

@article{citeulike:1628164, abstract = {BACKGROUNDThe complex molecular pathways governing implantation are unclear and ethical limitations limit studies in humans. Reversible histone acetylation regulates gene transcription and histone deacetylase inhibitors (HDACI) induce specific genes. Suberoylanilide hydroxamic acid (SAHA), a HDACI recently approved as an anti-cancer drug, induces the morphological and functional differentiation of human endometrial gland cells through up-regulation of glycodelin, a secretory phase dominant protein. METHODSWe investigated whether SAHA improves implantation in an in vitro implantation assay using the human endometrial adenocarcinoma cell line, Ishikawa and the choriocarcinoma cell line, JAR. RESULTSIn an in vitro implantation assay, JAR spheroids attached and adhered to Ishikawa cells in a time dependent manner. Glycodelin induction, following treatment with ovarian steroid hormones or SAHA, enhanced implantation. The improvement in implantation was also obtained when glycodelin was overexpressed without stimulation and was almost completely abrogated by glycodelin gene silencing. CONCLUSIONSThis study demonstrates that glycodelin is a key regulatory protein of implantation and suggests that SAHA may have a capacity to supplant steroid derivatives in the treatment of infertility. 10.1093/humrep/dem263}, author = {Uchida, Hiroshi and Maruyama, Tetsuo and Ohta, Kuniaki and Ono, Masanori and Arase, Toru and Kagami, Maki and Oda, Hideyuki and Kajitani, Takashi and Asada, Hironori and Yoshimura, Yasunori}, citeulike-article-id = {1628164}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/humrep/dem263}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17720699}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17720699}, day = {24}, doi = {10.1093/humrep/dem263}, journal = {Hum. Reprod.}, keywords = {cancer, epigenetics, histone, physiology, reproduction}, month = {August}, pages = {dem263+}, posted-at = {2007-09-06 18:52:28}, priority = {5}, title = {Histone deacetylase inhibitor-induced glycodelin enhances the initial step of implantation}, url = {http://dx.doi.org/10.1093/humrep/dem263}, year = {2007} }

TY - JOUR ID - citeulike:1628164 L3 - citeulike-article-id:1628164 N2 - BACKGROUNDThe complex molecular pathways governing implantation are unclear and ethical limitations limit studies in humans. Reversible histone acetylation regulates gene transcription and histone deacetylase inhibitors (HDACI) induce specific genes. Suberoylanilide hydroxamic acid (SAHA), a HDACI recently approved as an anti-cancer drug, induces the morphological and functional differentiation of human endometrial gland cells through up-regulation of glycodelin, a secretory phase dominant protein. METHODSWe investigated whether SAHA improves implantation in an in vitro implantation assay using the human endometrial adenocarcinoma cell line, Ishikawa and the choriocarcinoma cell line, JAR. RESULTSIn an in vitro implantation assay, JAR spheroids attached and adhered to Ishikawa cells in a time dependent manner. Glycodelin induction, following treatment with ovarian steroid hormones or SAHA, enhanced implantation. The improvement in implantation was also obtained when glycodelin was overexpressed without stimulation and was almost completely abrogated by glycodelin gene silencing. CONCLUSIONSThis study demonstrates that glycodelin is a key regulatory protein of implantation and suggests that SAHA may have a capacity to supplant steroid derivatives in the treatment of infertility. 10.1093/humrep/dem263 JF - Hum. Reprod. TI - Histone deacetylase inhibitor-induced glycodelin enhances the initial step of implantation SP - dem263 KW - cancer KW - epigenetics KW - histone KW - physiology KW - reproduction AU - Uchida, Hiroshi AU - Maruyama, Tetsuo AU - Ohta, Kuniaki AU - Ono, Masanori AU - Arase, Toru AU - Kagami, Maki AU - Oda, Hideyuki AU - Kajitani, Takashi AU - Asada, Hironori AU - Yoshimura, Yasunori PY - 2007/08/24/ UR - http://dx.doi.org/10.1093/humrep/dem263 ER -