MAPK target networks in Arabidopsis thaliana revealed using functional protein microarrays Export

@article{citeulike:4891141, abstract = {10.1101/gad.1740009 Signaling through mitogen-activated protein kinases (MPKs) cascades is a complex and fundamental process in eukaryotes, requiring MPK-activating kinases (MKKs) and MKK-activating kinases (MKKKs). However, to date only a limited number of MKK–MPK interactions and MPK phosphorylation substrates have been revealed. We determined which MKKs preferentially activate 10 different MPKs in vivo and used the activated MPKs to probe high-density protein microarrays to determine their phosphorylation targets. Our analyses revealed known and novel signaling modules encompassing 570 MPK phosphorylation substrates; these substrates were enriched in transcription factors involved in the regulation of development, defense, and stress responses. Selected MPK substrates were validated by in planta reconstitution experiments. A subset of activated and wild-type MKKs induced cell death, indicating a possible role for these MKKs in the regulation of cell death. Interestingly, MKK7- and MKK9-induced death requires , a known regulator of cell death induced during plant innate immunity. Our predicted MKK–MPK phosphorylation network constitutes a valuable resource to understand the function and specificity of MPK signaling systems.}, author = {Popescu, Sorina C. and Popescu, George V. and Bachan, Shawn and Zhang, Zimei and Gerstein, Mark and Snyder, Michael and Dinesh-Kumar, Savithramma P.}, citeulike-article-id = {4891141}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gad.1740009}, citeulike-linkout-1 = {http://genesdev.cshlp.org/content/23/1/80.abstract}, citeulike-linkout-2 = {http://genesdev.cshlp.org/content/23/1/80.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19095804}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19095804}, day = {1}, doi = {10.1101/gad.1740009}, journal = {Genes \& Development}, keywords = {cytoscape, interactome}, month = {January}, number = {1}, pages = {80--92}, posted-at = {2009-06-18 12:27:57}, priority = {2}, title = {MAPK target networks in Arabidopsis thaliana revealed using functional protein microarrays}, url = {http://dx.doi.org/10.1101/gad.1740009}, volume = {23}, year = {2009} }

TY - JOUR ID - citeulike:4891141 L3 - citeulike-article-id:4891141 N2 - 10.1101/gad.1740009 Signaling through mitogen-activated protein kinases (MPKs) cascades is a complex and fundamental process in eukaryotes, requiring MPK-activating kinases (MKKs) and MKK-activating kinases (MKKKs). However, to date only a limited number of MKK–MPK interactions and MPK phosphorylation substrates have been revealed. We determined which MKKs preferentially activate 10 different MPKs in vivo and used the activated MPKs to probe high-density protein microarrays to determine their phosphorylation targets. Our analyses revealed known and novel signaling modules encompassing 570 MPK phosphorylation substrates; these substrates were enriched in transcription factors involved in the regulation of development, defense, and stress responses. Selected MPK substrates were validated by in planta reconstitution experiments. A subset of activated and wild-type MKKs induced cell death, indicating a possible role for these MKKs in the regulation of cell death. Interestingly, MKK7- and MKK9-induced death requires , a known regulator of cell death induced during plant innate immunity. Our predicted MKK–MPK phosphorylation network constitutes a valuable resource to understand the function and specificity of MPK signaling systems. IS - 1 JF - Genes & Development EP - 92 TI - MAPK target networks in Arabidopsis thaliana revealed using functional protein microarrays VL - 23 SP - 80 KW - cytoscape KW - interactome AU - Popescu, Sorina AU - Popescu, George AU - Bachan, Shawn AU - Zhang, Zimei AU - Gerstein, Mark AU - Snyder, Michael AU - Dinesh-Kumar, Savithramma PY - 2009/01/01/ UR - http://dx.doi.org/10.1101/gad.1740009 ER -