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High-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples Export

Opt. Lett., Vol. 34, No. 1. (1 January 2009), pp. 79-81.

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diffraction-tomography

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We have developed a tomographic diffractive microscope, equipped with a fluorescence confocal scanner. We measure experimentally the lateral resolution using an edge method and by comparing tomographic images of the same samples with wide-field and laser scanning confocal microscopy images; a scanning electron microscope image serves as a reference. The experimental resolution is shown to be to about 130 nm, or λ/(3.5 NA). This instrument also permits one to measure 3D, complex index of refraction distributions, a quantity that is not accessible to conventional microscopes, and we show how this feature may be used to observe KCl crystals, absorption of which is very weak.


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