Activity-dependent regulation of h channel distribution in hippocampal CA1 pyramidal neurons. Export

@article{citeulike:1681721, abstract = {The hyperpolarization-activated cation current, Ih, plays an important role in regulating intrinsic neuronal excitability in the brain. In hippocampal pyramidal neurons, Ih is mediated by h channels comprised primarily of the hyperpolarization-activated cyclic nucleotide-gated channel (HCN) subunits, HCN1 and HCN2. Pyramidal neuron h channels within hippocampal area CA1 are remarkably enriched in distal apical dendrites, and this unique distribution pattern is critical for regulating dendritic excitability. We utilized biochemical and immunohistochemical approaches in organotypic slice cultures to explore factors that control h channel localization in dendrites. We found that distal dendritic enrichment of HCN1 is first detectable at postnatal day 13, reaching maximal enrichment by the third postnatal week. Interestingly, we found that an intact entorhinal cortex, which projects to distal dendrites of CA1, but not area CA3, is critical for the establishment and maintenance of distal dendritic enrichment of HCN1. Moreover, blockade of excitatory neurotransmission using TTX, CNQX or APV redistributed HCN1 evenly throughout the dendrite without significant changes in protein expression levels. Inhibition of CaMKII activity, but not p38 MAPK also redistributed HCN1 in CA1 pyramidal neurons. We conclude that activation of ionotropic glutamate receptors by excitatory temporoammonic pathway projections from the entorhinal cortex establishes and maintains the distribution pattern of HCN1 in CA1 pyramidal neuron dendrites by activating calcium/calmodulin-dependent protein kinase II (CaMKII)-mediated downstream signals.}, address = {Neurology, Northwestern University, Chicago, IL 60610.}, author = {Shin, Minyoung and Chetkovich, Dane M M.}, citeulike-article-id = {1681721}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M703736200}, citeulike-linkout-1 = {http://www.jbc.org/cgi/content/abstract/282/45/33168}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17848552}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17848552}, doi = {10.1074/jbc.M703736200}, issn = {0021-9258}, journal = {J Biol Chem}, keywords = {h-channel, hcn, hippocamp}, month = {September}, posted-at = {2009-02-18 10:21:03}, priority = {2}, title = {Activity-dependent regulation of h channel distribution in hippocampal CA1 pyramidal neurons.}, url = {http://dx.doi.org/10.1074/jbc.M703736200}, year = {2007} }

TY - JOUR ID - citeulike:1681721 L3 - citeulike-article-id:1681721 N2 - The hyperpolarization-activated cation current, Ih, plays an important role in regulating intrinsic neuronal excitability in the brain. In hippocampal pyramidal neurons, Ih is mediated by h channels comprised primarily of the hyperpolarization-activated cyclic nucleotide-gated channel (HCN) subunits, HCN1 and HCN2. Pyramidal neuron h channels within hippocampal area CA1 are remarkably enriched in distal apical dendrites, and this unique distribution pattern is critical for regulating dendritic excitability. We utilized biochemical and immunohistochemical approaches in organotypic slice cultures to explore factors that control h channel localization in dendrites. We found that distal dendritic enrichment of HCN1 is first detectable at postnatal day 13, reaching maximal enrichment by the third postnatal week. Interestingly, we found that an intact entorhinal cortex, which projects to distal dendrites of CA1, but not area CA3, is critical for the establishment and maintenance of distal dendritic enrichment of HCN1. Moreover, blockade of excitatory neurotransmission using TTX, CNQX or APV redistributed HCN1 evenly throughout the dendrite without significant changes in protein expression levels. Inhibition of CaMKII activity, but not p38 MAPK also redistributed HCN1 in CA1 pyramidal neurons. We conclude that activation of ionotropic glutamate receptors by excitatory temporoammonic pathway projections from the entorhinal cortex establishes and maintains the distribution pattern of HCN1 in CA1 pyramidal neuron dendrites by activating calcium/calmodulin-dependent protein kinase II (CaMKII)-mediated downstream signals. JF - J Biol Chem SN - 0021-9258 AD - Neurology, Northwestern University, Chicago, IL 60610. TI - Activity-dependent regulation of h channel distribution in hippocampal CA1 pyramidal neurons. KW - h-channel KW - hcn KW - hippocamp AU - Shin, Minyoung AU - Chetkovich, Dane M PY - 2007/09/11/ UR - http://dx.doi.org/10.1074/jbc.M703736200 ER -