Blue native PAGE. Export

@article{citeulike:1218298, abstract = {Blue native PAGE (BN-PAGE) can be used for one-step isolation of protein complexes from biological membranes and total cell and tissue homogenates. It can also be used to determine native protein masses and oligomeric states and to identify physiological protein-protein interactions. Native complexes are recovered from gels by electroelution or diffusion and are used for 2D crystallization and electron microscopy or analyzed by in-gel activity assays or by native electroblotting and immunodetection. In this protocol, we describe methodology to perform BN-PAGE followed by (i) native extraction or native electroblotting of separated proteins, or (ii) a second dimension of tricine-SDS-PAGE or modified BN-PAGE, or (iii) a second dimension of isoelectric focusing (IEF) followed by a third dimension of tricine-SDS-PAGE for the separation of subunits of complexes. These protocols for 2D and 3D PAGE can be completed in 2 and 3 days.}, address = {Molekulare Bioenergetik, Zentrum der Biologischen Chemie, Universit\"{a}tsklinikum Frankfurt, Theodor-Stern-Kai 7, Haus 26, D-60590 Frankfurt, Germany.}, author = {Wittig, I. and Braun, H. P. and Sch\"{a}gger, H.}, citeulike-article-id = {1218298}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nprot.2006.62}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17406264}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17406264}, doi = {10.1038/nprot.2006.62}, issn = {1750-2799}, journal = {Nature protocols}, keywords = {complexes, interactions, methods, preparation, purification, separation, stoichiometry}, number = {1}, pages = {418--428}, posted-at = {2008-06-30 09:05:18}, priority = {2}, title = {Blue native PAGE.}, url = {http://dx.doi.org/10.1038/nprot.2006.62}, volume = {1}, year = {2006} }

TY - JOUR ID - citeulike:1218298 L3 - citeulike-article-id:1218298 N2 - Blue native PAGE (BN-PAGE) can be used for one-step isolation of protein complexes from biological membranes and total cell and tissue homogenates. It can also be used to determine native protein masses and oligomeric states and to identify physiological protein-protein interactions. Native complexes are recovered from gels by electroelution or diffusion and are used for 2D crystallization and electron microscopy or analyzed by in-gel activity assays or by native electroblotting and immunodetection. In this protocol, we describe methodology to perform BN-PAGE followed by (i) native extraction or native electroblotting of separated proteins, or (ii) a second dimension of tricine-SDS-PAGE or modified BN-PAGE, or (iii) a second dimension of isoelectric focusing (IEF) followed by a third dimension of tricine-SDS-PAGE for the separation of subunits of complexes. These protocols for 2D and 3D PAGE can be completed in 2 and 3 days. IS - 1 JF - Nature protocols SN - 1750-2799 AD - Molekulare Bioenergetik, Zentrum der Biologischen Chemie, Universitätsklinikum Frankfurt, Theodor-Stern-Kai 7, Haus 26, D-60590 Frankfurt, Germany. EP - 428 TI - Blue native PAGE. VL - 1 SP - 418 KW - complexes KW - interactions KW - methods KW - preparation KW - purification KW - separation KW - stoichiometry AU - Wittig, I AU - Braun, HP AU - Schägger, H PY - 2006/// UR - http://dx.doi.org/10.1038/nprot.2006.62 ER -