Inhibition of Translational Initiation by Let-7 MicroRNA in Human Cells Export

@article{citeulike:312316, abstract = {MicroRNAs (miRNAs) are [\~{}]21-nucleotide-long RNA molecules regulating gene expression in multicellular eukaryotes. In metazoa, miRNAs act by imperfectly base-pairing with the 3' untranslated region of target messenger RNAs (mRNAs) and repressing protein accumulation by an unknown mechanism. We demonstrate that endogenous let-7 microribonucleoproteins (miRNPs) or the tethering of Argonaute (Ago) proteins to reporter mRNAs in human cells inhibit translation initiation. M7G-cap-independent translation is not subject to repression, suggesting that miRNPs interfere with recognition of the cap. Repressed mRNAs, Ago proteins, and miRNAs were all found to accumulate in processing bodies. We propose that localization of mRNAs to these structures is a consequence of translational repression.}, author = {Pillai, Ramesh S. and Bhattacharyya, Suvendra N. and Artus, Caroline G. and Zoller, Tabea and Cougot, Nicolas and Basyuk, Eugenia and Bertrand, Edouard and Filipowicz, Witold}, citeulike-article-id = {312316}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1115079}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16081698}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16081698}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/full/309/5740/1573}, day = {02}, doi = {10.1126/science.1115079}, journal = {Science}, keywords = {ncrna}, month = {September}, number = {5740}, pages = {1573--1576}, posted-at = {2008-02-26 10:05:06}, priority = {2}, title = {Inhibition of Translational Initiation by Let-7 MicroRNA in Human Cells}, url = {http://dx.doi.org/10.1126/science.1115079}, volume = {309}, year = {2005} }

TY - JOUR ID - citeulike:312316 L3 - citeulike-article-id:312316 N2 - MicroRNAs (miRNAs) are [~]21-nucleotide-long RNA molecules regulating gene expression in multicellular eukaryotes. In metazoa, miRNAs act by imperfectly base-pairing with the 3' untranslated region of target messenger RNAs (mRNAs) and repressing protein accumulation by an unknown mechanism. We demonstrate that endogenous let-7 microribonucleoproteins (miRNPs) or the tethering of Argonaute (Ago) proteins to reporter mRNAs in human cells inhibit translation initiation. M7G-cap-independent translation is not subject to repression, suggesting that miRNPs interfere with recognition of the cap. Repressed mRNAs, Ago proteins, and miRNAs were all found to accumulate in processing bodies. We propose that localization of mRNAs to these structures is a consequence of translational repression. IS - 5740 JF - Science EP - 1576 TI - Inhibition of Translational Initiation by Let-7 MicroRNA in Human Cells VL - 309 SP - 1573 KW - ncrna AU - Pillai, Ramesh AU - Bhattacharyya, Suvendra AU - Artus, Caroline AU - Zoller, Tabea AU - Cougot, Nicolas AU - Basyuk, Eugenia AU - Bertrand, Edouard AU - Filipowicz, Witold PY - 2005/09/02/ UR - http://dx.doi.org/10.1126/science.1115079 ER -