ARF1 controls the activation of the PI3K pathway to regulate EGF dependent growth and migration of breast cancer cells. Export

@article{citeulike:3500541, abstract = {Activation of intracellular signaling pathways by growth factors is one of the major causes of cancer development and progression. Recent studies have demonstrated that monomeric G proteins of the Ras family are key regulators of cell proliferation, migration, and invasion. Using an invasive breast cancer cell lines, we demonstrate that the ADP-ribosylation factor 1 (ARF1), a small GTPase classically associated with the Golgi, is an important regulator of the biological effects induced by epidermal growth factor (EGF). Here, we show that this ARF isoform is activated following EGF stimulation and that in MDA-MB-231 cells, ARF1 is found in dynamic plasma membrane ruffles. Inhibition of endogenous ARF1 expression results in the inhibition of breast cancer cell migration and proliferation. The underlying mechanism involves the activation of the phosphatidylinositol-3 kinase (PI3K) pathway. Our data demonstrate that depletion of ARF1 markedly impairs the recruitment of the PI3K catalytic subunit (p110a) to the plasma membrane, and the association of the regulatory subunit (p85a) to the activated receptor. These results uncover a novel molecular mechanism by which ARF1 regulates breast cancer cell growth and invasion during cancer progression.}, author = {Boulay, Pierre-Luc L. and Cotton, Mathieu and Melancon, Paul and Claing, Audrey}, citeulike-article-id = {3500541}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M803603200}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18990689}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18990689}, day = {6}, doi = {10.1074/jbc.M803603200}, issn = {0021-9258}, journal = {The Journal of biological chemistry}, keywords = {arf1, cancer, disease, in\_vivo, mechanism, membrane, signaling}, month = {November}, posted-at = {2008-11-10 08:13:31}, priority = {2}, title = {ARF1 controls the activation of the PI3K pathway to regulate EGF dependent growth and migration of breast cancer cells.}, url = {http://dx.doi.org/10.1074/jbc.M803603200}, year = {2008} }

TY - JOUR ID - citeulike:3500541 L3 - citeulike-article-id:3500541 N2 - Activation of intracellular signaling pathways by growth factors is one of the major causes of cancer development and progression. Recent studies have demonstrated that monomeric G proteins of the Ras family are key regulators of cell proliferation, migration, and invasion. Using an invasive breast cancer cell lines, we demonstrate that the ADP-ribosylation factor 1 (ARF1), a small GTPase classically associated with the Golgi, is an important regulator of the biological effects induced by epidermal growth factor (EGF). Here, we show that this ARF isoform is activated following EGF stimulation and that in MDA-MB-231 cells, ARF1 is found in dynamic plasma membrane ruffles. Inhibition of endogenous ARF1 expression results in the inhibition of breast cancer cell migration and proliferation. The underlying mechanism involves the activation of the phosphatidylinositol-3 kinase (PI3K) pathway. Our data demonstrate that depletion of ARF1 markedly impairs the recruitment of the PI3K catalytic subunit (p110a) to the plasma membrane, and the association of the regulatory subunit (p85a) to the activated receptor. These results uncover a novel molecular mechanism by which ARF1 regulates breast cancer cell growth and invasion during cancer progression. JF - The Journal of biological chemistry SN - 0021-9258 TI - ARF1 controls the activation of the PI3K pathway to regulate EGF dependent growth and migration of breast cancer cells. KW - arf1 KW - cancer KW - disease KW - in_vivo KW - mechanism KW - membrane KW - signaling AU - Boulay, Pierre-Luc AU - Cotton, Mathieu AU - Melancon, Paul AU - Claing, Audrey PY - 2008/11/06/ UR - http://dx.doi.org/10.1074/jbc.M803603200 ER -