Coatomer, Arf1p, and nucleotide are required to bud coat protein complex I-coated vesicles from large synthetic liposomes. Export

@article{citeulike:3801886, abstract = {Synthetic coat protein complex I (COPI)-coated vesicles form spontaneously from large ( approximately 300 nm in diameter), chemically defined liposomes incubated with coatomer, Arf1p, and guanosine 5'-[gamma-thio]triphosphate. Coated vesicles are 40-70 nm in diameter, approximately the size of COPI vesicles formed from native membranes. The formation of COPI-coated buds and vesicles and the binding of Arf1p to donor liposomes depends on guanosine 5'-[gamma-thio]triphosphate. In contrast to the behavior of the COPII coat, coatomer binds to liposomes containing a variety of charged or neutral phospholipids. However, the formation of COPI buds and vesicles is stimulated by acidic phospholipids. In the absence of Arf1p, coatomer binds to liposomes containing dioleoylphosphatidic acid as a sole acidic phospholipid to form large coated surfaces without forming COPI-coated buds or vesicles. We conclude that Arf1p-GTP and coatomer comprise the minimum apparatus necessary to create a COPI-coated vesicle.}, author = {Spang, A. and Matsuoka, K. and Hamamoto, S. and Schekman, R. and Orci, L.}, citeulike-article-id = {3801886}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9736713}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9736713}, day = {15}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {arf1, budding, coatomer, copi, interactions, liposome, membrane, pinching\_off, vesicle}, month = {September}, number = {19}, pages = {11199--11204}, posted-at = {2008-12-17 16:44:47}, priority = {2}, title = {Coatomer, Arf1p, and nucleotide are required to bud coat protein complex I-coated vesicles from large synthetic liposomes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9736713}, volume = {95}, year = {1998} }

TY - JOUR ID - citeulike:3801886 L3 - citeulike-article-id:3801886 N2 - Synthetic coat protein complex I (COPI)-coated vesicles form spontaneously from large ( approximately 300 nm in diameter), chemically defined liposomes incubated with coatomer, Arf1p, and guanosine 5'-[gamma-thio]triphosphate. Coated vesicles are 40-70 nm in diameter, approximately the size of COPI vesicles formed from native membranes. The formation of COPI-coated buds and vesicles and the binding of Arf1p to donor liposomes depends on guanosine 5'-[gamma-thio]triphosphate. In contrast to the behavior of the COPII coat, coatomer binds to liposomes containing a variety of charged or neutral phospholipids. However, the formation of COPI buds and vesicles is stimulated by acidic phospholipids. In the absence of Arf1p, coatomer binds to liposomes containing dioleoylphosphatidic acid as a sole acidic phospholipid to form large coated surfaces without forming COPI-coated buds or vesicles. We conclude that Arf1p-GTP and coatomer comprise the minimum apparatus necessary to create a COPI-coated vesicle. IS - 19 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 EP - 11204 TI - Coatomer, Arf1p, and nucleotide are required to bud coat protein complex I-coated vesicles from large synthetic liposomes. VL - 95 SP - 11199 KW - arf1 KW - budding KW - coatomer KW - copi KW - interactions KW - liposome KW - membrane KW - pinching_off KW - vesicle AU - Spang, A AU - Matsuoka, K AU - Hamamoto, S AU - Schekman, R AU - Orci, L PY - 1998/09/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9736713 ER -