Modeling experimental image formation for likelihood-based classification of electron microscopy data. Export

@article{citeulike:4170840, abstract = {The coexistence of multiple distinct structural states often obstructs the application of three-dimensional cryo-electron microscopy to large macromolecular complexes. Maximum likelihood approaches are emerging as robust tools for solving the image classification problems that are posed by such samples. Here, we propose a statistical data model that allows for a description of the experimental image formation within the formulation of 2D and 3D maximum-likelihood refinement. The proposed approach comprises a formulation of the probability calculations in Fourier space, including a spatial frequency-dependent noise model and a description of defocus-dependent imaging effects. The Expectation-Maximization-like algorithms presented are generally applicable to the alignment and classification of structurally heterogeneous projection data. Their effectiveness is demonstrated with various examples, including 2D classification of top views of the archaeal helicase MCM and 3D classification of 70S E. coli ribosome and Simian Virus 40 large T-antigen projections.}, author = {Scheres, S. H. and N\'{u}\~{n}ez-Ram\'{\i}rez, R. and G\'{o}mez-Llorente, Y. and San Mart\'{\i}n, C. and Eggermont, P. P. and Carazo, J. M.}, citeulike-article-id = {4170840}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.str.2007.09.003}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17937907}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17937907}, doi = {10.1016/j.str.2007.09.003}, issn = {0969-2126}, journal = {Structure (London, England : 1993)}, keywords = {complexes, conform\_change, heterogeneity, image\_processing, maxlike, methods, separation, software, structure}, month = {October}, number = {10}, pages = {1167--1177}, posted-at = {2009-03-13 12:15:56}, priority = {2}, title = {Modeling experimental image formation for likelihood-based classification of electron microscopy data.}, url = {http://dx.doi.org/10.1016/j.str.2007.09.003}, volume = {15}, year = {2007} }

TY - JOUR ID - citeulike:4170840 L3 - citeulike-article-id:4170840 N2 - The coexistence of multiple distinct structural states often obstructs the application of three-dimensional cryo-electron microscopy to large macromolecular complexes. Maximum likelihood approaches are emerging as robust tools for solving the image classification problems that are posed by such samples. Here, we propose a statistical data model that allows for a description of the experimental image formation within the formulation of 2D and 3D maximum-likelihood refinement. The proposed approach comprises a formulation of the probability calculations in Fourier space, including a spatial frequency-dependent noise model and a description of defocus-dependent imaging effects. The Expectation-Maximization-like algorithms presented are generally applicable to the alignment and classification of structurally heterogeneous projection data. Their effectiveness is demonstrated with various examples, including 2D classification of top views of the archaeal helicase MCM and 3D classification of 70S E. coli ribosome and Simian Virus 40 large T-antigen projections. IS - 10 JF - Structure (London, England : 1993) SN - 0969-2126 EP - 1177 TI - Modeling experimental image formation for likelihood-based classification of electron microscopy data. VL - 15 SP - 1167 KW - complexes KW - conform_change KW - heterogeneity KW - image_processing KW - maxlike KW - methods KW - separation KW - software KW - structure AU - Scheres, SH AU - Núñez-Ramírez, R AU - Gómez-Llorente, Y AU - San Martín, C AU - Eggermont, PP AU - Carazo, JM PY - 2007/10// UR - http://dx.doi.org/10.1016/j.str.2007.09.003 ER -