GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat. Export

@article{citeulike:4236624, abstract = {Formation of coated carrier vesicles, such as COPI-coated vesicles from the cis-Golgi, is triggered by membrane binding of the GTP-bound form of ADP-ribosylation factors. This process is blocked by brefeldin A, which is an inhibitor of guanine nucleotide exchange factors for ADP-ribosylation factor. GBF1 is one of the guanine nucleotide-exchange factors for ADP-ribosylation factor and is localized in the Golgi region. In the present study, we have determined the detailed subcellular localization of GBF1. Immunofluorescence microscopy of cells treated with nocodazole or incubated at 15 degrees C has suggested that GBF1 behaves similarly to proteins recycling between the cis-Golgi and the endoplasmic reticulum. Immunoelectron microscopy has revealed that GBF1 localizes primarily to vesicular and tubular structures apposed to the cis-face of Golgi stacks and minor fractions to the Golgi stacks. GBF1 overexpressed in cells causes recruitment of class I and class II ADP-ribosylation factors onto Golgi membranes. Furthermore, overexpressed GBF1 antagonizes various effects of brefeldin A, such as inhibition of membrane recruitment of ADP-ribosylation factors and the COPI coat, and redistribution of Golgi-resident and itinerant proteins. These observations indicate that GBF1 is involved in the formation of COPI-coated vesicles from the cis-Golgi or the pre-Golgi intermediate compartment through activating ADP-ribosylation factors.}, author = {Kawamoto, K. and Yoshida, Y. and Tamaki, H. and Torii, S. and Shinotsuka, C. and Yamashina, S. and Nakayama, K.}, citeulike-article-id = {4236624}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12047556}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12047556}, issn = {1398-9219}, journal = {Traffic (Copenhagen, Denmark)}, keywords = {arf1, arno, copi, gbf1, gef, golgi, membrane, protein, traffic}, month = {July}, number = {7}, pages = {483--495}, posted-at = {2009-03-30 12:37:02}, priority = {2}, title = {GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12047556}, volume = {3}, year = {2002} }

TY - JOUR ID - citeulike:4236624 L3 - citeulike-article-id:4236624 N2 - Formation of coated carrier vesicles, such as COPI-coated vesicles from the cis-Golgi, is triggered by membrane binding of the GTP-bound form of ADP-ribosylation factors. This process is blocked by brefeldin A, which is an inhibitor of guanine nucleotide exchange factors for ADP-ribosylation factor. GBF1 is one of the guanine nucleotide-exchange factors for ADP-ribosylation factor and is localized in the Golgi region. In the present study, we have determined the detailed subcellular localization of GBF1. Immunofluorescence microscopy of cells treated with nocodazole or incubated at 15 degrees C has suggested that GBF1 behaves similarly to proteins recycling between the cis-Golgi and the endoplasmic reticulum. Immunoelectron microscopy has revealed that GBF1 localizes primarily to vesicular and tubular structures apposed to the cis-face of Golgi stacks and minor fractions to the Golgi stacks. GBF1 overexpressed in cells causes recruitment of class I and class II ADP-ribosylation factors onto Golgi membranes. Furthermore, overexpressed GBF1 antagonizes various effects of brefeldin A, such as inhibition of membrane recruitment of ADP-ribosylation factors and the COPI coat, and redistribution of Golgi-resident and itinerant proteins. These observations indicate that GBF1 is involved in the formation of COPI-coated vesicles from the cis-Golgi or the pre-Golgi intermediate compartment through activating ADP-ribosylation factors. IS - 7 JF - Traffic (Copenhagen, Denmark) SN - 1398-9219 EP - 495 TI - GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat. VL - 3 SP - 483 KW - arf1 KW - arno KW - copi KW - gbf1 KW - gef KW - golgi KW - membrane KW - protein KW - traffic AU - Kawamoto, K AU - Yoshida, Y AU - Tamaki, H AU - Torii, S AU - Shinotsuka, C AU - Yamashina, S AU - Nakayama, K PY - 2002/07// UR - http://view.ncbi.nlm.nih.gov/pubmed/12047556 ER -