Similar subunit interactions contribute to assembly of clathrin adaptor complexes and COPI complex: analysis using yeast three-hybrid system. Export

@article{citeulike:5149512, abstract = {Clathrin adaptor protein (AP) complexes are heterotetramers composed of two large, one medium, and one small subunits. By exploiting the yeast three-hybrid system, we have found that an interaction between the two large subunits of the AP-1 complex, gamma-adaptin and beta1-adaptin, is markedly enhanced in the presence of the small subunit, sigma1. Similarly, two large subunits of the AP-4 complex, epsilon-adaptin and beta4-adaptin, are found to interact with each other only in the presence of the small subunit, sigma4. Furthermore, we have found that an interaction between two large subunits of the COPI F subcomplex, gamma-COP and beta-COP, is detectable only in the presence of zeta-COP. Because these COPI subunits have common ancestral origins to the corresponding AP subunits, these three-hybrid data, taken together with the previous two-hybrid data, suggest that the AP complexes and the COPI F subcomplex assemble by virtue of similar subunit interactions.}, author = {Takatsu, H. and Futatsumori, M. and Yoshino, K. and Yoshida, Y. and Shin, H. W. and Nakayama, K.}, citeulike-article-id = {5149512}, citeulike-linkout-0 = {http://dx.doi.org/10.1006/bbrc.2001.5081}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11409905}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11409905}, day = {22}, doi = {10.1006/bbrc.2001.5081}, issn = {0006-291X}, journal = {Biochemical and biophysical research communications}, keywords = {adaptors, coatomer, evolution, homology}, month = {June}, number = {4}, pages = {1083--1089}, posted-at = {2009-07-14 17:44:31}, priority = {2}, title = {Similar subunit interactions contribute to assembly of clathrin adaptor complexes and COPI complex: analysis using yeast three-hybrid system.}, url = {http://dx.doi.org/10.1006/bbrc.2001.5081}, volume = {284}, year = {2001} }

TY - JOUR ID - citeulike:5149512 L3 - citeulike-article-id:5149512 N2 - Clathrin adaptor protein (AP) complexes are heterotetramers composed of two large, one medium, and one small subunits. By exploiting the yeast three-hybrid system, we have found that an interaction between the two large subunits of the AP-1 complex, gamma-adaptin and beta1-adaptin, is markedly enhanced in the presence of the small subunit, sigma1. Similarly, two large subunits of the AP-4 complex, epsilon-adaptin and beta4-adaptin, are found to interact with each other only in the presence of the small subunit, sigma4. Furthermore, we have found that an interaction between two large subunits of the COPI F subcomplex, gamma-COP and beta-COP, is detectable only in the presence of zeta-COP. Because these COPI subunits have common ancestral origins to the corresponding AP subunits, these three-hybrid data, taken together with the previous two-hybrid data, suggest that the AP complexes and the COPI F subcomplex assemble by virtue of similar subunit interactions. IS - 4 JF - Biochemical and biophysical research communications SN - 0006-291X EP - 1089 TI - Similar subunit interactions contribute to assembly of clathrin adaptor complexes and COPI complex: analysis using yeast three-hybrid system. VL - 284 SP - 1083 KW - adaptors KW - coatomer KW - evolution KW - homology AU - Takatsu, H AU - Futatsumori, M AU - Yoshino, K AU - Yoshida, Y AU - Shin, HW AU - Nakayama, K PY - 2001/06/22/ UR - http://dx.doi.org/10.1006/bbrc.2001.5081 ER -