COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes. Export

@article{citeulike:5921491, abstract = {COPII vesicle formation requires only three coat assembly subunits: Sar1p, Sec13/31p, and Sec23/24p. PI 4-phosphate or PI 4,5-bisphosphate is required for the binding of these proteins to liposomes. The GTP-bound form of Sar1p recruits Sec23/24p to the liposomes as well as to the ER membranes, and this Sar1p-Sec23/24p complex is required for the binding of Sec13/31p. Ultrastructural analysis shows that the binding of COPII coat proteins to liposomes results in coated patches, coated buds, and coated vesicles of 50-90 nm in diameter. Budding proceeds without rupture of the donor liposome or vesicle product. These observations suggest that the assembly of the COPII coat on the ER occurs by a sequential binding of coat proteins to specific lipids and that this assembly promotes the budding of COPII-coated vesicles.}, author = {Matsuoka, K. and Orci, L. and Amherdt, M. and Bednarek, S. Y. and Hamamoto, S. and Schekman, R. and Yeung, T.}, citeulike-article-id = {5921491}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9568718}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9568718}, day = {17}, issn = {0092-8674}, journal = {Cell}, keywords = {budding, mechanism, vesicle}, month = {April}, number = {2}, pages = {263--275}, posted-at = {2009-10-11 16:48:17}, priority = {2}, title = {COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9568718}, volume = {93}, year = {1998} }

TY - JOUR ID - citeulike:5921491 L3 - citeulike-article-id:5921491 N2 - COPII vesicle formation requires only three coat assembly subunits: Sar1p, Sec13/31p, and Sec23/24p. PI 4-phosphate or PI 4,5-bisphosphate is required for the binding of these proteins to liposomes. The GTP-bound form of Sar1p recruits Sec23/24p to the liposomes as well as to the ER membranes, and this Sar1p-Sec23/24p complex is required for the binding of Sec13/31p. Ultrastructural analysis shows that the binding of COPII coat proteins to liposomes results in coated patches, coated buds, and coated vesicles of 50-90 nm in diameter. Budding proceeds without rupture of the donor liposome or vesicle product. These observations suggest that the assembly of the COPII coat on the ER occurs by a sequential binding of coat proteins to specific lipids and that this assembly promotes the budding of COPII-coated vesicles. IS - 2 JF - Cell SN - 0092-8674 EP - 275 TI - COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes. VL - 93 SP - 263 KW - budding KW - mechanism KW - vesicle AU - Matsuoka, K AU - Orci, L AU - Amherdt, M AU - Bednarek, SY AU - Hamamoto, S AU - Schekman, R AU - Yeung, T PY - 1998/04/17/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9568718 ER -