Highly specific gene silencing by artificial microRNAs in Arabidopsis. Export

@article{citeulike:801680, abstract = {Plant microRNAs (miRNAs) affect only a small number of targets with high sequence complementarity, while animal miRNAs usually have hundreds of targets with limited complementarity. We used artificial miRNAs (amiRNAs) to determine whether the narrow action spectrum of natural plant miRNAs reflects only intrinsic properties of the plant miRNA machinery or whether it is also due to past selection against natural miRNAs with broader specificity. amiRNAs were designed to target individual genes or groups of endogenous genes. Like natural miRNAs, they had varying numbers of target mismatches. Previously determined parameters of target selection for natural miRNAs could accurately predict direct targets of amiRNAs. The specificity of amiRNAs, as deduced from genome-wide expression profiling, was as high as that of natural plant miRNAs, supporting the notion that extensive base pairing with targets is required for plant miRNA function. amiRNAs make an effective tool for specific gene silencing in plants, especially when several related, but not identical, target genes need to be downregulated. We demonstrate that amiRNAs are also active when expressed under tissue-specific or inducible promoters, with limited nonautonomous effects. The design principles for amiRNAs have been generalized and integrated into a Web-based tool (http://wmd.weigelworld.org).}, author = {Schwab, Rebecca and Ossowski, Stephan and Riester, Markus and Warthmann, Norman and Weigel, Detlef}, citeulike-article-id = {801680}, citeulike-linkout-0 = {http://dx.doi.org/10.1105/tpc.105.039834}, citeulike-linkout-1 = {http://www.plantcell.org/cgi/content/abstract/18/5/1121}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16531494}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16531494}, day = {1}, doi = {10.1105/tpc.105.039834}, issn = {1040-4651}, journal = {The Plant cell}, month = {May}, number = {5}, pages = {1121--1133}, posted-at = {2009-11-06 05:39:28}, priority = {2}, title = {Highly specific gene silencing by artificial microRNAs in Arabidopsis.}, url = {http://dx.doi.org/10.1105/tpc.105.039834}, volume = {18}, year = {2006} }

TY - JOUR ID - citeulike:801680 L3 - citeulike-article-id:801680 N2 - Plant microRNAs (miRNAs) affect only a small number of targets with high sequence complementarity, while animal miRNAs usually have hundreds of targets with limited complementarity. We used artificial miRNAs (amiRNAs) to determine whether the narrow action spectrum of natural plant miRNAs reflects only intrinsic properties of the plant miRNA machinery or whether it is also due to past selection against natural miRNAs with broader specificity. amiRNAs were designed to target individual genes or groups of endogenous genes. Like natural miRNAs, they had varying numbers of target mismatches. Previously determined parameters of target selection for natural miRNAs could accurately predict direct targets of amiRNAs. The specificity of amiRNAs, as deduced from genome-wide expression profiling, was as high as that of natural plant miRNAs, supporting the notion that extensive base pairing with targets is required for plant miRNA function. amiRNAs make an effective tool for specific gene silencing in plants, especially when several related, but not identical, target genes need to be downregulated. We demonstrate that amiRNAs are also active when expressed under tissue-specific or inducible promoters, with limited nonautonomous effects. The design principles for amiRNAs have been generalized and integrated into a Web-based tool (http://wmd.weigelworld.org). IS - 5 JF - The Plant cell SN - 1040-4651 EP - 1133 TI - Highly specific gene silencing by artificial microRNAs in Arabidopsis. VL - 18 SP - 1121 AU - Schwab, Rebecca AU - Ossowski, Stephan AU - Riester, Markus AU - Warthmann, Norman AU - Weigel, Detlef PY - 2006/05/01/ UR - http://dx.doi.org/10.1105/tpc.105.039834 ER -