Imaging Cell Death with Radiolabeled Annexin V in an Experimental Model of Rheumatoid Arthritis Export

@article{citeulike:4364147, abstract = {Rheumatoid arthritis is associated with chronic synovial inflammation due to the abnormal accumulation of macrophages and autoreactive T lymphocytes in joints. The autoreactive cells cause an inflammatory proapoptotic response to self-antigens resulting in eventual bone, cartilage, and soft-tissue loss and destruction. The goal of our study was to determine the timing and intensity of apoptosis in joints using 99mTc-labeled annexin V, an in vivo marker of apoptosis, in a murine model of immune arthritis. Methods: We used 99mTc-annexin V and autoradiography to study the extent and severity of apoptosis in the front and rear paws of DBA/1 mice with type II collagen-induced rheumatoid arthritis. Results: Compared with control values (n = 10), there was a significant (P < 0.002) nearly 3-fold increase in uptake of 99mTc-annexin V in the front foot pads, rear toes, rear foot pads, and heels at the time of maximal extremity swelling as determined by serial caliper measurements at 4 wk after inoculation with type II bovine collagen (n = 9). The front toes had a 5- to 6-fold increase in uptake compared with control values (P < 0.001). Histologic analysis revealed only scattered rare lymphocytes in the periarticular soft tissues, without joint destruction. Dual autoradiography with 125I-bovine serum albumin as a control showed that 99mTc-annexin V localization was specific. Treatment with methylprednisolone for 1 wk (n = 8) at 4 wk after immunization with type II collagen decreased 99mTc-annexin V uptake by 3- to 6-fold compared with control values (P < 0.002). Conclusion: 99mTc-annexin V can detect collagen-induced immune arthritis and its response to steroid therapy before joint destruction.}, author = {Post, Anneke M. and Katsikis, Peter D. and Tait, Jonathan F. and Geaghan, Sharon M. and Strauss, William H. and Blankenberg, Francis G.}, citeulike-article-id = {4364147}, citeulike-linkout-0 = {http://jnm.snmjournals.org/cgi/content/abstract/43/10/1359}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12368374}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12368374}, day = {1}, journal = {J Nucl Med}, keywords = {rheumatoid-arthritis}, month = {October}, number = {10}, pages = {1359--1365}, posted-at = {2009-04-19 05:13:31}, priority = {2}, title = {Imaging Cell Death with Radiolabeled Annexin V in an Experimental Model of Rheumatoid Arthritis}, url = {http://jnm.snmjournals.org/cgi/content/abstract/43/10/1359}, volume = {43}, year = {2002} }

TY - JOUR ID - citeulike:4364147 L3 - citeulike-article-id:4364147 N2 - Rheumatoid arthritis is associated with chronic synovial inflammation due to the abnormal accumulation of macrophages and autoreactive T lymphocytes in joints. The autoreactive cells cause an inflammatory proapoptotic response to self-antigens resulting in eventual bone, cartilage, and soft-tissue loss and destruction. The goal of our study was to determine the timing and intensity of apoptosis in joints using 99mTc-labeled annexin V, an in vivo marker of apoptosis, in a murine model of immune arthritis. Methods: We used 99mTc-annexin V and autoradiography to study the extent and severity of apoptosis in the front and rear paws of DBA/1 mice with type II collagen-induced rheumatoid arthritis. Results: Compared with control values (n = 10), there was a significant (P < 0.002) nearly 3-fold increase in uptake of 99mTc-annexin V in the front foot pads, rear toes, rear foot pads, and heels at the time of maximal extremity swelling as determined by serial caliper measurements at 4 wk after inoculation with type II bovine collagen (n = 9). The front toes had a 5- to 6-fold increase in uptake compared with control values (P < 0.001). Histologic analysis revealed only scattered rare lymphocytes in the periarticular soft tissues, without joint destruction. Dual autoradiography with 125I-bovine serum albumin as a control showed that 99mTc-annexin V localization was specific. Treatment with methylprednisolone for 1 wk (n = 8) at 4 wk after immunization with type II collagen decreased 99mTc-annexin V uptake by 3- to 6-fold compared with control values (P < 0.002). Conclusion: 99mTc-annexin V can detect collagen-induced immune arthritis and its response to steroid therapy before joint destruction. IS - 10 JF - J Nucl Med EP - 1365 TI - Imaging Cell Death with Radiolabeled Annexin V in an Experimental Model of Rheumatoid Arthritis VL - 43 SP - 1359 KW - rheumatoid-arthritis AU - Post, Anneke AU - Katsikis, Peter AU - Tait, Jonathan AU - Geaghan, Sharon AU - Strauss, William AU - Blankenberg, Francis PY - 2002/10/01/ UR - http://jnm.snmjournals.org/cgi/content/abstract/43/10/1359 ER -